SUMO及SUMO化修饰蛋白在恶性胶质瘤发生中的作用

Effects of SUMO and proteins modified by SUMO on occurrence of malignant gliomas

目的:观察小泛素化相关修饰蛋白质类(SUMO)及SUMO化修饰蛋白在恶性胶质瘤组织中的表达,阐明SUMO化与恶性胶质瘤发生的关系。方法:人脑正常组织和恶性胶质瘤组织样品分别作为正常对照组和恶性胶质瘤组,采用蛋白免疫印迹法检测SUMO1、SUMO2、SUMO活化酶(Aos1和Uba2)、SUMO结合酶(UBC9)和SUMO连接酶(PIAS1、PIAS2、PIAS3和Pc2)蛋白的表达水平。结果:与正常对照组比较,恶性胶质瘤组织中SUMO1、SUMO2、Aos1、UBC9和PIAS3蛋白表达水平明显升高(P<0.01),Uba2、PIAS1和PIAS2蛋白表达水平明显降低(P<0.01)。结论:SUMO及SUMO化修饰蛋白可能对恶性胶质瘤的发生起重要的促进作用。

Objective： To observe the expressions of small ubiquitin-related modifier protein （SUMO） and related proteins modified by SUMO in the malignant gliomas tissue, and to explore the relationship between sumolation and the occurrence of malignant glioma. Methods： Two samples of normal brain tissues and malignant glioma tissue were prepared as control group and malignant glioma group. The expressions of SUMO1, SUMO2, Aosl, Uba2, UBCg, PIAS1, PIAS2, PIAS3 and Pc2 proteins were detected by Western blotting assay. Results： Compared with control group, the expression levels of SUMO1, SUMO2, Aosl, UBC9 and PIAS3 proteins in malignant glima group were significantly increased （P〈0. 01）, but the expression levels of Uba2, PIAS1 and PIAS2 proteins were decreased （P〈0.01）. Conclusion： SUMO and poteins modified by SUMO may play an important role in the occurrence of malignant gliomas.