摘要
目的:探讨厄洛替尼和山奈酚对表皮生长因子受体酪氨酸激酶(EGFR-TPK)的抑制作用,并阐明二者对卵巢癌细胞生长的协同抑制作用机制。方法:培养人卵巢癌SKOV-3细胞,将细胞分为空白组、山奈酚组和厄洛替尼组,将不同浓度山奈酚与厄洛替尼加入到EGFR-TPK和人卵巢癌SKOV-3细胞中,ELISA法检测EGFR-TPK的活性,采用Transwell小室法检测各组SKOV-3细胞侵袭转移能力,采用酶标仪检测各组SKOV-3细胞凋亡蛋白caspase-3活性,MTT法检测各组SKOV-3细胞的生长抑制率及山奈酚联合厄洛替尼对SKOV-3细胞的生长抑制作用。结果:山奈酚和厄洛替尼对EGFR-TPK的半数抑制浓度(IC50)分别为(2.33±0.32)g·L-1和(1.75±0.18)g·L-1,二者比较差异无统计学意义(P>0.05)。与空白组比较,山奈酚和厄洛替尼组穿膜细胞数减少(P<0.05或P<0.01),SKOV-3细胞中凋亡蛋白caspase-3活性明显增加(P<0.05或P<0.01)。随着山奈酚和厄洛替尼浓度增加,SKOV-3细胞生长抑制率不断增加,与空白组比较差异均有统计学意义(P<0.05或P<0.01)。结论:山奈酚联合厄洛替尼对人卵巢癌SKOV-3细胞具有协同抑制作用,并对SKOV-3细胞转移起到抑制作用。
Objective: To study the inhibitory effect of epidermal growth factor receptor tyrosine kinase (EGFR- TPK), and to clarify the mechanism of their synergetic inhibitory effects on the growth of ovarian cancer cells. Methods: The human ovarian cancer SKOV 3 cells were cultured and divided into blank group, kaempferol group and tarceva group. The activities of EGFR-TPK were detected by ELISA method. The invasion abilities of SKOV-3 cell lines were determined by Transwell assay. The caspase-3 activities of SKOV-3 cells were measured. The inhibitory rates of growth of SKOV-3 cells were determined using MTT method. Results The half inhibitory concentrations (IC50) of kaempferol and tarceva were (52.33±0.32) g · L-land (1.75±0.18) g · L-1 , there was no significant differences between them (P〈0.05). The inhibitory rates of growth of SKOV-3 cells were increased with the increasing of doses of kaempferol and tarceva, and there were significant differences compared with blank group (P〈0. 05 or P〈0.01). The caspase-3 activities of SKOV-3 cells of in kaempferol and tarceva groups were significantly increased compared with blank group (P〈0.05). Conclusion.. Kaempferol in combination with tarceva has a synergetic effect on the growth of ovarian cancer SKOV-3 cells.
出处
《吉林大学学报(医学版)》
CAS
CSCD
北大核心
2016年第1期31-35,共5页
Journal of Jilin University:Medicine Edition
基金
国家医学教育发展中心基金资助课题(2011-29-08-011)