酵素和生根壮苗剂对对开蕨组培苗生长的影响

Effects of Enzyme and Root and Seedling Promoter on Tissue Culture Seedling Growth of Phyllitis japonica

以2 a生对开蕨组培苗为试验材料,0.3%尿素+0.3%磷酸二氢钾为对照,对对开蕨组培苗喷施酵素和生根壮苗剂两种叶面肥,研究两种叶面肥对其生长的影响.结果表明:随着施肥次数的增加,各处理下对开蕨组培苗生长加快,长势增强.与对照相比,叶面施肥后处理6的叶片数量、叶面积增幅最大,差异达显著水平(P<0.05),处理3次之;处理5的株高、冠幅净增长量最大,差异显著(P<0.05);各处理的新萌发叶片数量和孢子囊叶片数量无明显差异;处理6的比叶重见效最快,施肥后期各处理无显著差异.综合考虑,生根壮苗剂600倍最有利于对开蕨组培苗的生长,其次是1 000倍花粉酵素+700倍光合酵素+10 000倍开根酵素.

Taking 0. 3% urea ＋ 0. 3% Potassium dihydrogen phosphate as contrast,two foliar fertilizers were designed and the influence of foliar fertilizers on growth of Phyllitis japonica tissue culture seedlings were investigated by the experiment. The results showed that Phyllitis japonica tissue culture seedlings not only accelerate growth but also enhance growing with the increase of the number of fertilization. Compared with the control,the leaf number,leaf area of treatment 6 had the largest increases after foliage dressing,the difference reached significant level（ P 0. 05）,followed by treatment 3. The plant height and crown diameter of treatment 5net income was the biggest,it shows significant difference（ P 0. 05）. The new bourgeon leaf number and sporangium leaf number of all treatments had no significant changes. The effect of specific leaf weight of treatment6 is obvious,but all treatments had no significant changes in the late period of fertilization. Comprehensively,the most conducive to growth of Phyllitis japonica was root and seedling promoter 600 times,the second was 1 000 times flowering enzyme bacterial fertilizer ＋ 700 times photosynthetic enzyme bacterial fertilizer ＋ 10 000 times open the root enzyme bacterial fertilizer.