FOXP3在人肺腺癌细胞中的表达意义

Clinical Value of FOXP3 Expression in Human Lung Adenocarcinoma Cells

目的通过检测FOXP3在人肺腺癌细胞中的表达情况,探讨FOXP3在人肺腺癌发生、发展中的作用机制.方法收集临床肺腺癌患者术后石蜡包埋标本42例和正常肺组织10例,采用免疫组化SP法检测FOXP3蛋白的表达水平,分析其在肺癌细胞组与正常支气管黏膜组表达的差异,以及其在肺癌细胞的表达与临床病理指标的内在联系.结果 FOXP3在人肺腺癌细胞高表达,阳性率为64.3%;在正常肺组织低表达,阳性率为20.0%,两组比较差异具有统计学意义(P=0.011).FOXP3蛋白表达与有无淋巴结转移显著相关,有淋巴结转移组FOXP3的阳性表达率为83.3%,无淋巴结转移组FOXP3的阳性表达率为50.0%,两组比较差异具有统计学意义(P=0.026).FOXP3蛋白表达与患者性别、年龄、肿瘤的大小和分化程度均无明显相关性(P>0.05).结论FOXP3参与人肺腺癌的发生、发展,可作为评判肺腺癌恶性生物学行为的指标之一.

Objective To explore the role and clinical value of FOXP3 in lung adenocarcinoma,by detecting the expression of FOXP3 protein in the tumor cells of primary lung adenocarcinoma. Method A total of 42 cases specimens from the operated patients with primary lung adenocarcinoma were selected in this study with 15 cases of normal lung tissue as control. FOXP3 expression was detected by immunohistochemistry technique（ SP method） and the relationship between FOXP3 expression and clinicopathological features were analyzed.Results The positive expression rate of FOXP3 was 64. 3% in lung adenocarcinoma,which was significantly higher than that of the control group with 20. 0%（ P = 0. 011）. FOXP3 expression in lung adenocarcinoma was closely associated with lymph node metastasis. The positive rate of FOXP3 in the lymph node metastasis group（ 83. 3%） was significantly higher than that of the non-lymph node metastasis group（ 50. 0%）（ P = 0. 026）.While no correlation was found between the expression of FOXP3 and the patients＇ gender,age,tumor size and differentiation（ P 0. 05）. Conclusion FOXP3 may play an important role in the tumorigenesis and development of lung adenocarcinoma. Therefore,FOXP3 could be used as one of the markers to evaluate themalignant biological behavior of lung adenocarcinoma.