片仔癀对部分肝切除小鼠的肝再生的作用研究

Effect study of Pien Tze Huang on liver regeneration after partial hepatectomy in mice

目的:研究片仔癀对70%肝切除小鼠肝再生的影响,为临床肝再生用药研究提供动物模型.方法:将8周龄雄性C57BL/6小鼠随机分为实验组(片仔癀预处理组)和对照组(生理盐水预处理组).采用70%肝切除方法建立肝再生模型,术前连续两周通过灌胃给予小鼠片仔癀0.2 mg/g体质量(实验组)和生理盐水(对照组).第14天灌胃完片仔癀和生理盐水2 h后对两组小鼠分别进行70%肝切除手术(PH).用肝质量/体质量比、实时荧光定量聚合酶链式反应(Real Time PCR)、丙氨酸转氨酶(ALT)和免疫组化等研究方法来评估片仔癀对小鼠部分肝切除手术后肝再生的影响.结果:与对照组相比,70%肝切除手术后48、72、168 h,实验组肝质量/体质量比降低,差异具有统计学意义(P<0.05);术后12、24、48、72 h,实验组血清ALT水平升高,差异具有统计学意义(P<0.05);术后12、24、48、72、168 h,实验组肝细胞生长因子(HGF)降低;术后48 h,实验组Ki-67表达量低于对照组.结论:片仔癀对70%肝切除小鼠的肝再生产生抑制作用,延缓了肝再生过程.

AIM： To explore the influence of Pien Tze Huang on liver regeneration after 70% PH and provide an animal model for clinical investigation of drugs for liver regeneration.METHODS：The C57 BL/6 mice were randomly divided into experimental group and control group.Mice in the control group were given 0.9%saline by intragastric administration,while the mice in the experimental group were given 0.2 mg / g body weight Pien Tze Huang for two weeks before 70% PH.A C57 BL /6 mice model of liver regeneration were established by 70% hepatectomy.Mice were all subjected to 70% PH two hours later at day 14.The influence of Pien Tze Huang on liver regeneration were assessd by liver weight / body weight ratio,real-time PCR,ALT activity detection and immunochemical staining of Ki-67.RESULTS： At 48,72 and 168 h after70% PH,the liver weight / body weight ratio in the experimental group decreased when compared with the control group,with statistically significant difference（ P〈0.05）; At 12,24,48,72 h after PH,ALT levels in serum in the experimental group increased, and the difference was statistically significant（ P〈0.05）; At 12,24,48,72 and 168 h after PH,HGF level of experimental group was significantly lower than the control group,and the difference was statistically significant（ P〈0.05）;At 48 h after PH,the Ki-67 expression of the experimental group was significantly less than the control group,and the difference was statistically significant（ P〈0.05）.CONCLUSION： Pien Tze Huang can obviously inhibit liver regeneration on mice after 70%partial hepatectomy,which delays regeneration process of liver.