HBeAg、p53及Ki-67在肝癌患者血清和组织中的表达及其意义

Expression and significance of HBeAg,p53 and Ki-67 in hepatocellular carcinoma

目的探讨血清HBeAg和肝癌组织中p53、Ki-67的表达情况及其临床意义。方法应用酶联免役吸附试验（ELISA）检测57例肝癌患者及27例正常对照者血清HBeAg，免疫组织化学方法检测57例肝癌组织及27例癌旁肝组织芯片中p53、Ki-67的表达情况，并探讨肝癌患者3项指标之间的关系及其与肝癌临床病理参数间的相关性。结果肝癌患者和正常对照者血清中HBeAg表达比较差异无统计学意义（P〉0．05）；肝癌组织中p53和Ki-67表达较癌旁组织增高，差异有统计学意义（P均〈0．01）；在57例患者中，HBeAg阳性者25例，占43．9％；p53阳性44例，占77．2％；HBeAg阳性同时伴有p53突变者23例。HBeAg的表达与肿瘤分期、p53阳性表达及Ki．67表达有关（P均〈0．05）；p53突变与肿瘤分化程度、分期及Ki-67表达相关（P均〈0．05）。结论肝癌患者HBeAg阳性、p53突变及Ki-67表达增高在肝癌的发展中起到协同作用。

Objective To explore the level and clinical significance of HBeAg in serum, of p53 and Ki-67 in hepatocellular carcinoma（HCC） tissuesand. Method ELISA was used to detect the level of HBeAg in serum of 57 HCC patients and 27control cases. The expression of p53 and Ki-67 in tissue chips of 57 cases of HCC and 27 cases of paratumor tissues were examined by immunohistochemical staining. Correlation of these indicators and clinicopathological parameters in HCC patients were discussed. Result There was no significant difference in the serum HBeAg level between HCC patients and controls（ P 〉 O. 05 ） ;while the expression of p53 and Ki-67 in HCC tissues were higher than those of the paratumor tis- sues （ P 〈 0.01 ） ; of all 57 HCC patients,25 cases （43.9 % ） were HBeAg positive and 44 cases （ 77.2% ） were p53 positive ;meanwhile ,23 patients were positive in both HBeAg and p53. HBeAg expression was related to tumor stage, positive p53 and Ki-67 expression（ P 〈 0.05 ） ; p53 mutation was associated with tumor differentiation,stage and Ki-67 expression （ P 〈 0.05 ）. Conclusion Positive HBeAg expression, p53 mutation and increased expression of Ki-67 may mutually promote the development of HCC.