Doublecortin样激酶1在结肠肿瘤中的表达及意义

Expression and Significance of Doublecortin-Like Kinase 1 in Colorectal Neoplasia

目的研究Doublecortin样激酶1（DCLK1）在结肠肿瘤组织中的表达及与结肠癌发生发展的关系。方法采用免疫组织化学方法检测20例结肠癌旁正常黏膜组织、20例结肠腺瘤、60例结肠癌及20例结肠癌转移灶组织中DCLK1的表达，分析结肠癌组织中DCLK1的表达与临床病理因素的关系。结果DCLK1在结肠癌旁正常黏膜组织、结肠腺瘤组织、结肠癌组织及结肠癌转移灶组织中的阳性表达率分别为20．0％、70．0％、65．0％和60．0％。结肠癌组织中DCLK1蛋白表达与临床分期和淋巴结转移密切相关，而与患者的性别、年龄、肿瘤大小及肿瘤的组织学分级无相关性。结论DCLK1的过表达可能在结肠肿瘤的发展及转移中起重要的作用。

Objective To investigate the expression of doublecortin-like kinase 1 （DCLK1） in colorectal neoplasia and its correlation with the occurrence and development of colonic cancer. Methods The expression of DCLK1 was determined by immunohistochemical staining in 20 adjacent normal tissues, 20 colon adenoma, 60 colon carcinoma tissues and 20 corresponding metastases. The expression of DCLK1 in the biological behavior of colorectal cancer was analyzed. Results The positive expression rates of DCLK1 in adjacent normal tissues, colon adenoma tissues, colon carcinoma tissues and corresponding metastases tissues were 20.0%, 70.0%, 65.0% and 60.0%. Expression of DCLK1 was correlated with TNM stage and lymph node metastasis, but had no correlation with gender, age, magnitude of the tumor and histological grade. Conclusion The over expression of DCLK1 may play an important role in the development and metastasis of colorectal neoplasia.