实验性大鼠脾气虚证模型的建立及指标检测

Establishment and evaluation of the experimental rat model of spleen-qi deficiency syndrome

目的建立实验性大鼠脾气虚证模型,为相关中药药理学研究提供实验依据。方法采用劳倦伤脾加饮食失节多因素复合法建立中医脾气虚证大鼠模型,并通过体征变化、白细胞计数、淋巴细胞数量、红细胞计数和血红蛋白含量分析、胸腺指数和脾脏指数、D-木糖吸收实验等对所建模型进行评价。结果与对照组比,模型组脾气虚证大鼠体质量减少,毛色枯槁,泄泻;白细胞数、淋巴细胞数、血红蛋白含量、胸腺指数和脾脏指数较对照组均显著降低;脾气虚证大鼠的小肠吸收功能有所减弱。结论实验性脾气虚证模型建立成功,可用于相关中药的药理学研究。白细胞数和淋巴细胞数可作为判定脾气虚证动物模型的灵敏指标。

Objective To establish the rat model of spleen-qi deficiency syndrome for the study of Chinese medicine pharmacology. Methods Rat models were established by the method of multi factors intervention,namely fatigue combined dietary restriction. The variations of physical sign,the number of white blood cells,lymphocytes and red blood cells,hemoglobin content,thymus and spleen indexes and D-xylose absorption of this model were evaluated. Results Compared with the control group,spleen-qi deficiency rats showed the syndrome of loss body mass,dry hair color,thin and diarrhea. The number of white blood cells and lymphocytes,hemoglobin contents,and thymus and spleen indexes were significantly lower than the control group. D-xylose absorption test showed that the absorption function of small intestine in model group was lower than that of the control group. Conclusion The rat model of spleen-qi deficiency syndrome has been successfully established. It could be used for the study of Chinese medicine pharmacology. The number of white blood cells and lymphocytes could be used as a sensitive index for this model.