藁本内酯对AngⅡ诱导的A7r5细胞迁移的影响

Effect of Ligustilide on A7r5 cell migration induced by AngⅡ

目的研究藁本内酯抑制AngⅡ诱导的A7r5细胞迁移的有效浓度及时间。方法采用MTT比色法测定藁本内酯对细胞的毒性;将A7r5细胞铺在6孔板并进行培养,待密度达到70%~80%后,用细胞划痕实验和Transwell细胞迁移实验评估藁本内酯对AngⅡ诱导的A7r5细胞迁移能力的变化,不同浓度（0、2.5、5、10μg/m L）的藁本内酯作用于A7r5细胞,于0、6、12、24 h观察细胞迁移情况,拍照记录,计算细胞迁移速度。结果藁本内酯对AngⅡ诱导的A7r5细胞迁移有明显的抑制作用,在0~10μg/m L浓度范围内呈剂量依赖关系,浓度越高其抑制细胞迁移越明显。同一浓度不同作用时间藁本内酯对AngⅡ诱导的A7r5细胞迁移抑制作用差异具有统计学意义（P〈0.05）;同一时间不同浓度的藁本内酯对AngⅡ诱导的A7r5细胞迁移抑制作用差异具有统计学意义（P〈0.05）。结论藁本内酯能抑制A7r5细胞的迁移,终质量浓度为10μg/m L藁本内酯作用于1μg/m L AngⅡ诱导的A7r5细胞12 h,其对A7r5细胞迁移抑制作用最明显。

Objective To investigate the inhibitory effect of concentration and time of Ligustilide on the migration of vascular smooth muscle A7r5 cells induced by Ang Ⅱ. Methods The cell cytotoxicity was assessed by MTT method. A7r5 cells were planted and cultured in 6-well plate. When A7r5 cells at 70%-80% confluency,the cell migration was measured by wound healing and Transwell migration assay. The cells were treated with 0,2. 5,5 and 10 μg/m L Ligustilide. A7r5 cell migration was observed under microscope at 0,6,12 and 24 h. The rate of cell migration was calculated. Results Ligustilide inhibited A7r5 cell migration induced by AngⅡ,and its inhibitory effect on the migration velocity was concentrationdependent in 0 ~ 10 μg/m L. The same concentration of Ligustilide at different times showed different inhibitory effects on A7r5 cell migration（ P 〈0.05）. The different concentrations of Ligustilide at the same time displayed different inhibitory effects on A7r5 cell migration（ P 〈0. 05）. Conclusion Ligustilide can prevent the vascular smooth muscle A7r5 cell migration induced by Ang Ⅱ. The inhibitory effect of Ligustilide on A7r5 cell migration is most obvious under the final concentration of 10 μg/m L at 12 h.