大鼠心脏骤停后血栓弹力图及单核细胞组织因子表达变化的研究

The Changes of Thrombelastography and Monocyte Tissue Factor in a Rat Model of Cardiac Arrest

目的:研究心跳骤停大鼠恢复自主循环后凝血的变化及其机制。方法:通过致颤法建立大鼠心肺复苏模型,恢复自主循环的雄性SD大鼠18只随机平均分为3组(n=6),假手术组,心肺复苏半小时组,心肺复苏1小时组,分别于相应时间点(复苏后半小时,1小时)采血,行血栓弹力图监测,并分离血液单核细胞,检测单核细胞组织因子mRNA水平的表达变化。结果:与对照组相比复苏后半小时组血栓弹力图R值明显缩短(P<0.05);与对照组相比,复苏后半小时及1小时组单核细胞组织因子表达明显增加(P<0.05)。结论 :TEG快速的监测出心肺复苏后早期凝血系统激活;单核细胞组织因子表达增加是心肺复苏后早期凝血激活的原因之一。

Objective ：The aim of this study was to characterize coagulation changes during cardiac arrest and post-resuscitation. Methods ： The Sprague-Dwaley rats were randomly divided into three groups（n =6）, shamoperation group, ROSC 30 min group, ROSC 1h group. We performed to detect TEG, TF-m RNA level of mononuclear leucocytes. The TF-m RNA levels of mononuclear leucocytes were measured by Q-PCR. Results ： TEG R time were prolonged in ROSC 30 min group compared with sham-operation group（P〈0.05）. The TF-m RNA level of mononuclear leucocytes were increased in ROSC 30 min group and ROSC1 h group compared with sham-operation group（P〈0.05）. Conclusion： TEG rapid monitoring of abnormal blood coagulation after cardiopulmonary resuscitation.Increased expression of single nuclear cell tissue factor is one of the causes of abnormal coagulation mechanism in the early stage of cardiopulmonary resuscitation.