PTPRN2和Ki-67在肝细胞肝癌中的表达及临床意义

Clinical significance of expression of receptor-type tyro sine-protein phosphatase N2 and proliferation cell nuclear antigen in hepatocellular carcinoma

目的:探究受体型蛋白酪氨酸磷酸酶N2(receptor-type tyrosine-protein phosphatase N2,PTPRN2)和增殖细胞核抗原(proliferation cell nuclear antigen,Ki-67)在肝细胞肝癌(hepatocellular carcinoma,HCC)中的表达与临床参数的相关性及其临床意义.方法:收集郑州大学第一附属医院HCC标本186例及其相应的癌旁组织(adjacent normal liver tissue,ANLT)180例、远癌组织138例,运用免疫组织化学染色的方法检测蛋白PTPRN2和Ki-67在HCC、ANLT、远癌组织的表达情况.结果:在186例HCC患者中,蛋白PTPRN2与Ki-67阳性表达率分别为55.9%、49.5%,PTPRN2的表达与血清甲胎蛋白(alpha fetoprotein,AFP)含量、肿瘤大小、临床分期、组织学分化程度相关,有统计学意义(P<0.05);Ki-67的表达与淋巴结转移、临床分期、组织学分化程度相关,有统计学意义(P<0.05);PTPRN2和Ki-67的表达无明显相关性(r=﹣0.161,P>0.05).结论:PTPRN2和Ki-67的高表达与HCC发展进程密切相关,但两者间无明显相关性.

AIM： To explore the correlation of receptor- type tyrosine-protein phosphatase N2 （PTPRN2） and proliferation cell nuclear antigen （Ki-67） expression with clinical parameters in hepatocellular carcinoma （HCC）.METHODS： We collected 186 HCC specimens from patients treated at the First Affiliated Hospital of Zhengzhou University, 180 tumor adjacent normal liver tissues, and 138 non-neoplastic distant tissues. The expression of PTPRN2 and Ki-67 proteins was examined by immunohistochemistry in the above tissues. RESULTS： The positive expression rates of PTPRN2 and Ki-67 were 55.9% and 49.5%, respectively. The positive expression of PTPRN2 was correlated with serum AFP level, tumor size, TNM stage, and histopathological differentiation （P 〈 0.05）. The expression of Ki-67 was correlated with lymph node metastasis, TNM stage, and histopathological differentiation （P 〈 0.05）. There was no significant correlation between PTPRN2 and Ki-67 expression （r = -0.161, P 〉 0.05）. CONCLUSION： The high expression of PTPRN2 and Ki-67 is closely associated with HCC development, but there is no significant correlation between them.