FAT10 mRNA在肝癌和结肠癌患者血浆中的表达及诊断价值

Diagnostic value of plasma HLA-F adjacent transcript 10 mRNA expression in patients with hepatocellular carcinoma or colon cancer

目的:探索血浆中人类白细胞抗原FAT10(humanleukocy teantigen-Fadjacent transcript 10,HLA-FAT10)m RNA表达水平,评估其对肝癌和结肠癌的诊断价值.方法:收集70例肝癌、60例肝硬化、64例结肠癌和64例健康对照者的血浆,应用实时荧光定量PCR(RT-PCR)和酶联吸附试验(E L I S A)法检测FAT10 mRNA和甲胎蛋白(a-fetoprotein,AFP)蛋白的表达水平.采用SPSS21.0统计软件进行数据分析.结果:FAT10 mRNA在肝癌、肝硬化、健康对照组中的阳性表达率分别为72.85%,28.3%和12.5%,三者之间差异显著(P<0.05),其高表达与肝癌TNM分期及淋巴结转移有关(P<0.05).FAT10 mRNA在结肠癌的阳性表达率明显高于健康对照组(P<0.05),其上调表达与结肠癌Duke's分期和远处转移相关(P<0.05).在AFP阴性肝癌中FAT10 m RNA的诊断灵敏度为66.6%.小肝癌与非肝癌组(肝硬化和健康组)比较时,FAT10 mRNA的灵敏度为68.8%,特异度为79.8%,联合AFP蛋白后,诊断灵敏度提高至84.4%.结论:FAT10 m RNA有可能成为肝癌和结肠癌诊断中的一个新的血清学指标,且可以应用于AFP阴性肝癌的辅助诊断,联合FAT10 mRNA和AFP蛋白可以提高肝癌的诊断率.

AIM： To detect plasma levels of human leukocyte antigen （HLA）-F adjacent transcript 10 （FAT10） mRNA in patients with hepatocellular carcinoma （HCC） or colon cancer （CC）, and to assess its diagnostic value.METHODS： Blood samples were collected from 70 patients with HCC, 60 patients with cirrhosis, 64 patients with CC, and 64 healthy volunteers. The expression levels of plasma FAT10 mRNA and α-fetoprotein （AFP） protein were measured by RT-PCR and ELISA, respectively.RESULTS： The positive rates of FAT10 mRNA in plasma of patients with HCC, those with cirrhosis and healthy controls were 72.85%, 28.3% and 12.5%, respectively, and there were significantly differences in the three groups （P 〈 0.05）. High expression levels of FAT10 mRNA in HCC were related to TNM stage and lymph node metastasis （P 〈 0.05）. The positive rate of FAT10 mRNA was also significantly higher in CC patients than in healthy controls （P 〈 0.05）. The up-regulation of FAT10 mRNA in CC was closely related to Duke＇s stage and distancemetastasis. FAT10 mRNA had a sensitivity of 66.6% for diagnosis of AFP-negative HCC. Furthermore, the FAT10 mRNA had a sensitivity of 68.8% and specificity of 79.8% in distinguishing small HCC patients from non- HCC individuals （cirrhosis patients and healthy controls）. Combined AFP and FAT10 mRNA detection could improve the sensitivity to 84.4%. CONCLUSION： FAT10 mRNA is a promising serological marker for HCC and CC. Plasma FAT10 mRNA and AFP protein could be combined to improve the diagnosis of HCC.