荜茇宁对急性胰腺炎大鼠干扰素-γ及硫氧还蛋白过氧化物酶-4基因的表达影响

Effect of piperlonguminine on expression of peroxiredoxin-4 gene in rats with acute pancreatitis

目的:探讨荜茇宁对胰腺组织硫氧还蛋白过氧化物酶-4(peroxiredoxin-4,Prdx-4)及炎症介质干扰素-γ(interferon-γ,IFN-γ)表达的影响机制.方法:30只SD大鼠按数字表法随机分成假手术(S)组,模型[重症急性胰腺炎(severe acute pancreatitis,SAP)]组,荜茇宁(P)组,每组10只.采用逆行胰胆管注射5%的牛黄胆酸钠(0.1mL/100 g)建立SAP模型,SO组以生理盐水代替牛黄胆酸钠,P组于建模后立即将荜茇宁注入大鼠腹腔(5 mg/kg),12h后处死大鼠留取标本,大鼠胰腺组织干燥箱烤至恒重后检测干湿比、荧光定量PCR检测Prdx-4mRNA表达、HE染色观察病理学变化、紫外分光光度计检测血清淀粉酶(amylase,AMS)及ELISA法检测血清IFN-γ变化.结果:3组大鼠胰腺组织干湿比、血清AMS和IFN-γ浓度及胰腺组织Prdx-4 mRNA相对表达量比较,SO组和P组均比SAP组明显降低,但P组比SO组增高,差异均具有统计学意义(P<0.05);病理评分P组比SAP组(3.86分±1.24分vs 8.24分±1.67分,P<0.05)降低,但较SO组高(P<0.05).结论:荜茇宁可降低SAP大鼠血清AMS和IFN-γ水平及下调胰腺组织中Prdx-4 mRNA表达量,改善胰腺病理损伤,推测荜茇宁可能通过抗炎作用改善SAP病情.

AIM： To observe the effect of piperlonguminine on the expression of peroxidoxin-4 （Prdx-4） in pancreatic tissue and serum levels of inflammatory mediators.METHODS： Thirty SD rats were randomly divided into a sham operated group （SOgroup）, an SAP group and a piperlonguminine group （P group） with 10 rats in each group. A rat acute pancreatitis model was established by retrograde injection of 5% sodium tauroeholate （0.1 mL/100 g） into the biliopancreatic duct. Sodium tauroeholate was replaced with saline in the SO group. Piperlonguminine （5 mg/kg） was injected intraperitoneally in the P group after model development. The rats were sacrificed 12 h after operation, and pancreatic tissue samples were taken to detect the tissue wet/dry ratio, the expression level of Prdx-4 mRNA and histopathological changes. Serum levels of amylase （AMS） and interferon-γ（IFN-γ） were also measured. RESULTS： The wet/dry ratio, AMS, IFN-γ and Prdx-4 mRNA in the SO group were significantly lower than those in the SAP group, but these indexes in the P group were significantly higher than those in the SO group （P 〈 0.05）. The pancreatic histopathologic score in the P group was lower than that of the SAP group （3.86 ± 1.24 vs 8.24 ± 1.67, P 〈 0.05）, but higher than that of the SO group （P 〈 0.05）. CONCLUSION： Piperlonguminine can not only reduce the levels of AMS, IFN-γ and Prdx-4 mRNA but also improve pancreatic pathological damage. Piperlonguminine alleviates pancreatitis via an anti-inflammatory effect.