桑白皮化学拆分工艺及互不交叉性研究

Splitted Fractions and Unoverlapping Analysis of Chemical Constituents of Cortex Mori

目的:建立桑白皮全成分的拆分工艺,并对各拆分组分进行互不交叉性研究与工艺稳定性考察。方法:首先采用水煎、萃取、醇沉、大孔吸附树脂等技术建立桑白皮化学组分的拆分方法;然后运用HPLC-PDA、HPLC-ELSD色谱法与Chem Pattern化学计量学软件相结合,采用主成分分析法与聚类分析法探讨各拆分组分是否有交叉。结果:桑白皮的全成分拆分为5个组分,将不同检测器的HPLC色谱数据结合化学计量学分析,结果显示各拆分组分互不交叉,该工艺所得10批次各拆分组分,通过HPLC-PDA特征图谱研究,进行相似度评价,工艺稳定。结论:本研究建立了稳定的桑白皮化学组分拆分工艺,该工艺稳定、重现性良好,各个拆分组分基本无化学成分交叉。

Objective： Toestablished the split method of nature and flavor material foudition of Cortex Mori,analysis of Cortex Mori chemical characteristics of the split components by HPLC,validae the unoverlapping analysis of Chemical Constituents of Cortex Mori in chemometrics methods. Methods： Established Cortex Mori chemical composition of the split method by steam distillation,extraction,alcohol,macroporous adsorption resin technology. With the combined applications of steam distillation,water extraction and alcohol precipitation,liquid-liquid extraction and column chromatography over macroporous resin,thesplitted-fractions method of the chemical constituents of poriacocos was established; Unoverlapping Analysis of Chemical Constituents of Cortex Mori by combining the HPLC-PDA and HPLC-ELSD in Chem Patternclustering analysis and principal component analysis. Results： We splitted the material basis of decoction of Cortex Mori for 5 groups,each group compositions substantially free of chemical components intersect. The process is stable and controllable by 10 batch in similarity evaluation. Conclusion： The split technology of nature and flavor material foundation of Cortex Mori is stable and practical,Indicated that Cortex Mori resolvability constituent holo-composition which was detached uncross as each one could be well separated under no supervision mode.