阿霉素所致大鼠肾病综合征肾小球固定阴离子位点的研究

CHANGES OF GLOMERULAR FIXED ANIONIC CHARGE SITES IN ADRIAMYCIN NEPHROSIS IN RATS

按5mg/kg剂量给大白鼠一次性静脉注射阿霉素,引起肾病综合征,用胶体铁染色显示其不同病期肾小球固定阴离子位点数量的变化,用图像分析仪定量分析。结果表明:用药后3小时肾小球固定阴离子位点巳明显丢失;阴离子位点丢失较蛋白尿出现早,且蛋白尿越严重,丢失越多。提示肾病综合征蛋白尿的产生机理可能与肾小球固定阴离子位点的丢失有关。

A single intravenous injection of adri-aycin (5mg per kg.) into rats inducednephrosis. In the various periods of themodel, alterations of glomerular anionicsites stained with colloidal iron wereanalyzed quantitatively by image analyzerfor the first time at home and abroad.Theresults showed that there wa a significantdecrease in glomerular anionic charge sitesat 3 hours after administration of adriamy-cin as compared with that of the controlanimals (P<0.01). Marked loss of theanionic charge sites preceded the onset ofproteinuria and was steadily increasingalong with progress of the disease. Theresults suggest that loss of glomerularanionic charge sites may play an importantrole in the development of proteinuria innephrotic syndrome.