不同前处理及显色方法测定粗多糖含量的研究

Analysis of Determination of Crude Polysaccharide Content with Different Pre-treatment and Colorimetric Methods

目的:探讨不同前处理方法、蒽酮硫酸显色法和苯酚硫酸显色法对粗多糖分析结果的影响。方法:以粗多糖的含量为评价指标,对灵芝孢子粉、乌药精粉应用不同的提取方法和测定方法进行分析。结果:不同的前处理方法测定灵芝孢子粉、乌药精粉中的粗多糖的含量不同;相同前处理下,采用苯酚硫酸显色法和蒽酮硫酸显色法,样本中粗多糖的含量无差异。结论:不同样本需采用不同的前处理,否则会出现样本粗多糖含量过高或过低现象;蒽酮硫酸显色法和苯酚硫酸显色法所测粗多糖线性、稳定性较好,适合大多数样品的检测。

OBJECTIVE： To probe into the effect of different pre-treatment methods and different determination methods like anthrone-sulfuric acid method and phenol-sulfuric acid method on the analysis of crude polysaccharide.METHODS： The content of crude polysaccharide was set as the evaluation index,different extraction methods and determination methods of ganoderma lucidum spores powder and lindera aggregata flavor powder were studied.RESULTS： Different contents of crude polysaccharide were determined in ganoderma lucidum spores powder and lindera aggregata flavor powder by different pre-treatment methods; to adopt the phenol-sulfuric acid method and phenol-sulfuric acid method with the same pre-treatment methods,there were no difference of the contents of crudepolysaccharide in the samples. CONCLUSIONS： Different samples need to adopt different pre-treatment methods,otherwise the determined contents of crude polysaccharide would be too high or too low in the samples; phenol-sulfuric acid method and anthrone-sulfuric acid anthrone method in determination of crude polysaccharide has good linear correlation and stability,which is suitable for most detection of the samples.