蝴蝶兰“大辣椒”组织培养与快速繁殖

Tissue Culture and Rapid Propagation of Big Chili Phalaenopsis

采用蝴蝶兰品种"大辣椒"幼嫩花梗作为试验材料,通过正交试验设计研究基本培养基、植物生长调节剂成分及含量对花梗不定芽的诱导、增殖、分化、生根的影响。结果表明,不定芽诱导的最佳培养基为:花宝一号+NAA 0.3mg·L^(-1)+6-BA 5mg·L^(-1);不定芽增殖和分化的最佳培养基为:花宝一号+NAA 0.2mg·L^(-1)+6-BA6mg·L^(-1),增殖系数最高达到5.53倍;最佳的壮苗和生根培养基为1/2MS+NAA 0.2mg·L^(-1),生根率为96%,椰子汁、苹果汁、土豆汁作为复合添加物对生根较为有利;组培生根苗移栽成活率在91.5%以上。

The young,tender flower stalks of the Big Chili Phalaenopsis were used for this study on the tissue culture and rapid propagation of the orchid.Using an orthogonal design experiment,the effects of the basic culture medium as well as hormone application and concentration on the pedicelinduction,proliferation and differentiation of the adventitious buds and the seedling development and rooting of the plant were investigated.The results showed that（a）the best medium for inducing the adventitious buds was Hyponex No.1＋NAA（0.3mg·L^-1）＋6-BA（5mg·L^-1）;（b）the best medium for the proliferation and differentiation of the adventitious buds was Hyponex No.1＋NAA（0.2mg·L^-1）＋6-BA（6mg·L^-1）,which resulted in a multiplication factor up to 5.53times;（c）the best medium forseedling development and rooting was 1/2MS＋NAA（0.2mg·L^-1）,which yielded a rooting rate of 96%,and coconut milk,apple juice,potato juice as parts of the composite additives were found to be advantageous for rooting;and（d）the survival rate of the transplanted tissue culture was greater than 91.5%.