不同局部肿胀麻醉液对体外培养人脂肪干细胞增殖与成脂分化的影响

Effect of different tumescent local anesthesia on the proliferation and differentiation of human adipose stem cells cultured in vitro

背景:获取脂肪术中需要应用不同的局部肿胀麻醉液,其可能会对细胞生物特性产生一定的影响。目的:探讨体外培养条件下不同局部肿胀麻醉液对人脂肪干细胞增殖与成脂分化的影响。方法:取第3代人脂肪干细胞分别用布比卡因、罗哌卡因、利多卡因局部肿胀麻醉液进行培养,对照组用低糖DMEM培养基进行培养,检测各组干预不同时间人脂肪干细胞增殖活性、细胞上清乳酸脱氢酶活性和成脂分化比例。结果与结论:不同局部肿胀麻醉液干预后1,2,3,4,5 d细胞上清乳酸脱氢酶活性值显著高于对照组(P<0.05),布比卡因组显著高于罗哌卡因组、利多卡因组(P<0.05)。各局部肿胀麻醉液组细胞生长吸光度值均低于对照组(P<0.05),且布比卡因组显著低于罗哌卡因组、利多卡因组(P<0.05)。各组人脂肪干细胞成脂分化比例差异无显著性意义(P>0.05)。结果表明,局部肿胀麻醉液(布比卡因、罗哌卡因、利多卡因)均能够抑制人脂肪干细胞增殖,但不会对细胞成脂分化产生明显的影响。

BACKGROUND： Different local anesthetic solutions for tumescent liposuction are necessary and have a certain effect on the biological characteristics of human adipose stem cells. OBJECTIVE： To explore the effect of different tumescent local anesthesia on the proliferation and differentiation of human adipose stem cells cultured in vitro. METHODS： Passage 3 human adipose stem cells were intervened by different tumescent local anesthesia（bupivacaine, ropivacaine, lidocaine）. Cells cultured in low-glucose DMEM served as controls. Proliferation ability, lactate dehydrogenase activity in the supernatant and percentage of cells under adipogenic differentiation were detected. RESULTS AND CONCLUSION： After intervention for 1, 2, 3, 4, 5 days, the supernatant lactate dehydrogenase activity in the bupivacaine, ropivacaine, lidocaine groups was significantly higher than that in the control group（P〈0.05）, and moreover, the lactate dehydrogenase activity in the bupivacaine group was significantly higher than that in the ropivacaine group and lidocaine group（P〈0.05）. The absorbance value of human adipose stem cells was lower in the bupivacaine, ropivacaine, lidocaine groups than the control group（P〈0.05） as well as lower in the bupivacaine group than the ropivacaine and lidocaine groups（P〈0.05）. There was no significant difference in the percentage of cells under adipogenic differentiation between groups（P〉0.05）. Overall, these findings indicate that different tumescent local anesthesia（bupivacaine, ropivacaine, lidocaine） can all inhibit the proliferation of human adipose stem cells, but have no certain effects on the adipogenic differentiation.