内源性增加单个核细胞自体移植提升血管闭塞性脉管炎调节性T细胞比例

Autologous peripheral blood mononuclear cell transplantation following cell mobilization increases CD4^+CD25^+ regulatory T cells in rats with thromboangiitis obliterans

背景:自体外周造血干细胞具有多向分化潜能的特点,可促进缺血肢体新生血管形成,改善和恢复患肢的血液供应。目的:观察自体外周血单个核细胞自体移植对血管闭塞性脉管炎大鼠的治疗作用。方法:将24只大鼠随机分为对照组、模型组和粒细胞集落刺激因子组。在模型组和粒细胞集落刺激因子组大鼠中股动脉注射月桂酸钠建立血管闭塞性脉管炎大鼠模型。粒细胞集落刺激因子组大鼠连续5 d以重组人粒细胞集落刺激因子动员外周血单个核细胞,第7天接受外周血单个核细胞移植,对照组和模型组大鼠仅给予生理盐水。结果与结论:与造模前相比,模型组和粒细胞集落刺激因子组大鼠造模后足背部体温及CD4^+CD25^+调节性T细胞比例显著降低,下肢溃疡面积明显增加;粒细胞集落刺激因子组大鼠动员后第6天白细胞数量及CD34^+细胞占单个核细胞的百分比增加,外周血单个核细胞移植之后1个月时足背部体温与建模前接近,CD4^+CD25^+调节性T细胞比例显著上升。提示外周血单个核细胞内源性增加后进行自体移植,可提升血管闭塞性脉管炎大鼠调节性T细胞比例,有助于迅速缓解症状、促进溃疡愈合。

BACKGROUND： Autologous peripheral blood stem cells have pluripotent characteristics, promote ischemic limb angiogenesis, improve and restore the blood supply to the affected limb. OBJECTIVE： To observe the therapeutic effect of autologous peripheral blood mononuclear cell transplantation in rats with thromboangiitis obliterans. METHODS： Twenty-four rats were randomized into control, model and granulocyte colony stimulating factor（G-CSF） groups. In the latter two groups, animal models of thromboangiitis obliterans were established by femoral arterial injection of sodium laurate. In the G-CSF group, rats were injected with recombinant human G-CSF for 5 successive days for peripheral blood mononuclear cell mobilization, and then underwent peripheral blood mononuclear cell transplantation at day 7. In the control and model groups, rats were given normal saline injection. RESULTS AND CONCLUSION： After modeling, foot dorsum temperature and the percentage of CD4^＋CD25^＋ regulatory T cells decreased significantly in the model and G-CSF groups, and the area of leg ulcers was enlarged notably in these two groups. In the G-CSF group, the number of white cells and percentage of CD34^＋ cells to mononuclear cells were increased at 6 days after mobilization; the foot dorsum temperature and the percentage of CD4^＋CD25^＋ regulatory T cells increased significantly at 1 month after peripheral blood mononuclear cell transplantation, which were close to the normal values. These findings indicate that autologous peripheral blood mononuclear cell transplantation following cell mobilization increases the percentage of CD4^＋CD25^＋ regulatory T cells in rats with thromboangiitis obliterans, thus promoting relief of symptoms and ulcer healing.