灯盏细辛联合骨髓间充质干细胞移植对脑梗死的保护作用机制

Fleabane combined with bone marrow mesenchymal stem cell transplantation for cerebral infarction

背景:通过细胞移植重建损伤脑组织成为治疗脑梗死的新途径,骨髓间充质干细胞成为近年来细胞移植治疗领域的重要种子细胞之一。目的:探讨灯盏细辛注射液联合骨髓间充质干细胞移植对急性脑梗死大鼠S100B蛋白及超氧化物歧化酶表达的影响。方法:采用线栓法制作大鼠急性脑梗死模型,建模成功后将80只SD大鼠随机分为对照组、灯盏细辛组、骨髓间充质干细胞组及联合组。分别于治疗前后用酶联免疫法检测各组大鼠血清S100B蛋白水平,黄嘌呤氧化酶法检测各组大鼠血清超氧化物歧化酶的表达;通过NIHSS神经功能评分观察模型大鼠的神经行为学变化,通过TTC染色测定脑梗死体积。结果与结论:在治疗后第3,7,14天,灯盏细辛组、骨髓间充质干细胞组的S100B蛋白水平明显低于对照组,但高于联合组,差异有显著性意义(P<0.05);灯盏细辛组、骨髓间充质干细胞的超氧化物歧化酶表达水平明显高于对照组,低于联合组,差异有显著性意义(P<0.05);治疗后第1,2,3周各组的NIHSS神经功能评分比较,联合组<灯盏细辛组及骨髓间充质干细胞组<对照组,差异有显著性意义(P<0.05);在治疗后2周联合组的脑梗死体积明显小于灯盏细辛组及骨髓间充质干细胞组,灯盏细辛组及骨髓间充质干细胞组又明显小于对照组,差异有显著性意义(P<0.05)。结果表明灯盏细辛注射液联合骨髓间充质干细胞移植能够抑制急性脑梗死大鼠S100B蛋白表达,提高超氧化物歧化酶活性,从而起到脑保护作用。

BACKGROUND： Cell transplantation becomes a new approach for treatment of cerebral infarction. In recent years, bone marrow mesenchymal stem cells（BMSCs） have become an important kind of seed cells in cell transplantation. OBJECTIVE： To investigate the effect of fleabane injection combined with BMSC transplantation on S100 B protein and superoxide dismutase expression in acute cerebral infarction rats. METHODS： Animal models of acute cerebral infarction were made in Sprague-Dawley rats using suture method. After successful modeling, 80 model rats were randomly divided into control group, fleabane group, BMSC group and combined group（fleabane combined with BMSC transplantation）. Changes of serum S100 B protein and serum superoxide dismutase levels were detected using enzyme-linked immunosorbent assay and xanthine oxidase method, respectively, before and after treatment. NIHSS neurological function scores were measured to observe neurological behavior changes in model rats. The infarct volume was measured by TTC staining. RESULTS AND CONCLUSION： At 36, 7, 14 days after treatment, S100 B protein levels in the fleabane group and BMSC group were significantly lower than that in the control group, but higher than that in the combined group（P〈0.05）; serum superoxide dismutase levels in the fleabane group and BMSC group were significantly higher than that in the control group, but lower than that in the combined group（P〈0.05）. At 1, 2, 3 weeks after treatment, NIHSS neurological function scores were ranked as follows： combined group fleabane group and BMSC group control group, and there was a significant difference between groups（P〈0.05）. At 2 weeks after treatment, the infarct volume in the fleabane group and BMSC group was higher than that in the combined group but lower than that in the control group（P〈0.05）. These findings indicate that fleabane combined with BMSC transplantation can inhibit the expression of S100 B protein in rats with acute cerebral infarction, and promote the activity of superoxide dismutase, thereby playing a neuroprotective role.