柿鞣质水提取液对牙周膜细胞增殖的影响

Influence of persimmon tannin water extract on periodontal ligament fibroblast proliferation

背景:柿果实中含有丰富的鞣质类物质,鞣质具有抗菌、抗病毒、抗氧化、抗肿瘤、保护黏膜及收敛等多重种功效,其在口腔领域的实用性和安全性已得到多方面的认证。目的:观察不同浓度的柿鞣质水提取液对牙周膜细胞增殖的影响。方法:将青少年因正畸需要拔除的前磨牙放置于无菌的含有DMEM培养基中,体外培养牙周膜细胞。对全柿果实进行超声提取,牙周膜细胞与不同质量浓度(0.013 3,0.025 9,0.038 9,0.088,0.147,0.440,0 g/L)的柿鞣质水条件培养液分别培养24,48,72 h后,采用MTT法、考马斯亮蓝法和碱性磷酸酶法分别观察不同质量浓度柿鞣质水提取液在不同时间点对牙周膜细胞数量、细胞总蛋白合成以及细胞活性的影响。结果与结论:在24,48,72 h 3个时间点,各质量浓度柿鞣质水提取液(0.013 3,0.025 9,0.038 9,0.08 8,0.147及0.440 g/L)均能促进人牙周膜细胞数量增长,促进蛋白质的合成,并使得细胞活性增强,且效应呈浓度依赖性和时间依赖性。结果提示柿鞣质水提取液具有促进人牙周膜细胞增殖的作用。

BACKGROUND： Persimmon is rich in tannin substances. Tannin has antibacterial, antiviral, antioxidant, anti-tumor effects as well as protects the mucosa and has a role of convergence, and its practicality and safety in the mouth have been certified. OBJECTIVE： To investigate the effects of persimmon tannin water extract with different concentrations on the proliferation of periodontal ligament cells. METHODS： Premolars from adolescents due to orthodontic extraction were placed in a sterile DMEM medium to culture periodontal ligament cells in vitro. Tannin from persimmon fruit was extracted by ultrasound. Cells were cultured in medium containing different concentrations of persimmon tannin water extracts（0.013 3, 0.025 9, 0.038 9, 0.088, 0.147, 0.440, 0 g/L） for 24, 48 and 72 hours, respectively. Then, MTT, coomassie brilliant blue staining and alkaline phosphatase method were used to observe the effects of persimmon tannin water extract on the number of periodontal ligament cells, total protein content and cell viability at different time points, respectively. RESULTS AND CONCLUSION： At 24, 48 and 72 hours after culture, the persimmon tannin water extract at different concentrations could promote the cell growth, accelerate the protein synthesis, and strengthen the activity in a dose- and time-dependent manner. These findings indicate that the persimmon tannin waterextraction can promote the proliferation of human periodontal ligament cells.