摘要
目的 探讨检测子宫颈上皮内瘤变（CIN）中高危型人乳头瘤病毒（HPV）-DNA、p16INK4a（p16）及Ki-67蛋白表达的价值，为CIN的早期诊断、早期治疗提供理论依据。方法 将35例CINⅠ、44例CINⅡ、20例CINⅢ组织制成切片，用杂交捕获二代法（HC2法）检测HPV-DNA，用免疫组织化学MaxVision法检测p16和Ki-67蛋白的表达，结合临床病理因素进行分析。结果 HPV-DNA在CINⅠ、CINⅡ、CINⅢ中的阳性表达率分别为31.4 %（11／35）、63.6 %（28／44）、85.0 %（17／20）。p16在CINⅠ、CINⅡ、CINⅢ中的阳性表达率分别为65.7 %（23／35）、97.7 %（43／44）、100.0 %（20／20），三组间差异有统计学意义（χ^2＝21.30，P＝0.00）；随着p16阳性表达率的升高，鳞状上皮HPV感染程度加深。Ki-67蛋白在CINⅠ、CINⅡ、CINⅢ中的阳性表达率分别为77.1 %（27／35）、79.5 %（35／44）、95.0 %（19／20），其阳性表达率随着子宫颈鳞状上皮HPV感染程度加深而升高，但三组间差异无统计学意义（χ^2＝3.00，P＝0.22）。结论 HPV的感染与子宫颈鳞状上皮内瘤变的发生、发展相关。p16和Ki-67联合检测可作为CIN诊断的客观参考指标。
Objective To investigate the expression of HPV-DNA and p16, Ki-67 proteins in intraepithelial lesion of cervix （CIN） so as to provide the aluable evidence for the early clinical diagnosis and genetic therapy of the CIN. Methods Hybrid capture2 was used to detect HPV DNA in 35 cases of CINⅠ, 44 cases of CINⅡ, and 20 cases of CINⅢ. Immunohistochemical MaxVision was used to research the expression of p16 and Ki-67 protein in CINⅠ, CINⅡ and CINⅢ. The Results were analyzed with clinicopathological factors. Results Positive expression rates of HPV-DNA were 31.4 % （11/35）, 63.6 % （28/44）, 85.0 % （17/20） in CINⅠ, CINⅡ and CINⅢ, respectively. The expression rates of p16 in the three groups were 65.7 % （23/35）, 97.7 % （43/44）, 100.0 % （20/20）, there were statistically significant difference （χ^2 = 21.30, P = 0.00）. The expression rates of Ki-67 in the three groups were 77.1 % （27/35）, 79.5 % （35/44）, 95.0 % （19/20）, there were no difference （χ^2 = 3.00, P = 0.22）. Conclusions HPV infection is closely related to the occurrence and development of CIN. p16, Ki-67 can be used as objective marks in diagnosis of CIN.
出处
《肿瘤研究与临床》
CAS
2015年第12期823-826,共4页
Cancer Research and Clinic
