阳极氧化法改善钛表面的生物活性和生物相容性

Improvement of surface bioactivity and biocompatibility on titanium by anodic oxidation

采用阳极氧化法在钛金属表面成功制备了均匀的纳米结构,生成的纳米管内径约70 nm,壁厚20 nm,管长200 nm.通过模拟体液浸泡实验对植入体的生物活性进行了评价,并通过体外细胞培养实验对生物相容性进行了评价.结果表明:经模拟体液浸泡4周后,阳极氧化处理后的钛表面可诱导磷灰石生成,而仅经过机械打磨的钛金属表面不能.细胞培养实验表明,阳极氧化处理后的钛相较于未处理的钛,有利于细胞在其表面粘附和增殖.基于上述研究结果可知:阳极氧化处理后的钛表面,提高了表面的粗糙度和亲水性,从而改善了钛的生物活性和生物相容性.

Anodic oxidation was applied to produce a homogeneous and uniform array of nanotubes on the surface of titanium（Ti） implants. The inner diameter of the nanotubes was about 70 nm, the wall thickness was around 20 nm, and the depth was about 200 nm. The bioactivity of the Ti implants was evaluated by simulated body fluid soaking test. The biocompatibility was investigated by vitro cell culture test. The results showed that bone-like apatite was formed on the anodized Ti surface, but not on the as-polished Ti surface after immersion in simulated body fluid for four weeks. Cells cultured on the anodized Ti surface showed enhanced cell adhesion and proliferation, also presented an up-regulated gene expression of osteogenic markers OPN and OCN, compared to those cultured on the as-polished Ti surface. Based on these results, it can be concluded that anodic oxidation improved the bioactivity and biocompatibility of Ti surface, which was attributed to the formation of nanostructured surface as well as the nanosurface inducing high surface roughness and hydrophilicity.