聚集素在认知障碍患者脑脊液及外周血中的表达

Clusterin expression in cerebrospinal fluid and peripheral blood of cognitive impairment patients

目的分析认知障碍患者脑脊液中聚集素（Clusterin）水平与p淀粉样蛋白1—42（Aβ1-42）水平的相关性，并探讨外周血聚集素作为阿尔茨海默病（AD）及遗忘型轻度认知功能障碍（aMCI）生物标志物的可能性。方法收集76例认知障碍患者的脑脊液，应用ELISA法测定脑脊液中Aβ1-42和聚集素水平，进行相关分析；另外收集125例AD患者、124例aMCI患者及116名健康对照（NC）的外周血，应用ELISA法测定血清聚集素水平，对3组研究对象血清聚集素的差异进行协方差分析。结果脑脊液中Aβ1-42水平与聚集素水平呈正相关（r=0．256，P〈0．05）。AD、aMCI和Nc3组间血清聚集素水平差异无统计学意义FAD组（36．84±1．40）ug／ml，aMCI组（34．94±1．22）uu／ml，NC组（37．38±1．13）ug／ml，P〉0．05]。结论认知障碍患者脑脊液中Aβ1-42水平与聚集素水平呈正相关，但外周血聚集素尚不足以作为AD及aMCI诊断的生物标志物。

Objective To analyze the correlation of clusterin and Aβ1-42 level in CSF in patients with cognitive impairment, and to explore the possibility of serum clusterin as a biomarker for Alzheimer disease（AD） and amnestic mild cognitive impairment（aMCI）. Methods Totals of 76 cognitive impairment patients were first included in this study and then CSF was collected. The level of clusterin and Aβ1-42in CSF was evaluated by ELISA. The peripheral blood of 125 AD patients, 124 aMCI patients and 116 normal controls（NC） was also collected, detected serum clusterin level by ELISA. Covariation anal- ysis was applied to compare the level of serum clusterin among the three groups. Results The level of Aβ1-42 in CSF was positively correlated with clusterin （r = 0. 256 ,P = 0. 046）. There was no significant difference in serum clusterin levels among AD[（36.84＋1.40）/lg/ml], aMCI[（34.94＋1.22）ug/ml] and NC[（37.38＋ 1.13）ug/ml] （P 〈0.05） groups. Conclusions A[31-42 level in CSF is positively correlated with the level of clusterin in patients with cognitive impairment. However, serum clusterin is not evi- denced to be a biomarker for AD and aMCI.