纳米硫化镉对人肾小管上皮细胞氧化损伤的影响

Influence of nano-cadmium sulfide on oxidative damage of human kidney cell

目的探讨纳米硫化镉(n Cd S)对人肾小管上皮细胞(HKC)氧化损伤的影响及其机制。方法首先应用噻唑蓝(MTT)实验观察暴露于生理盐水(对照组)和不同剂量浓度纳米Cd S(处理组)处于对数生长期的人肾小管波细胞(HKC)存活率的影响。检测细胞内的丙二醛上(MDA)、谷胱甘肽(GSH)、谷胱甘肽过氧化物还原酶(GSH-Px)和超氧化歧化酶(SOD)含量,并采用蛋白质印迹法观察纳米Cd S对细胞的NF-κB蛋白的表达情况。结果随着n Cd S对HKC细胞染毒剂量的增加,细胞存活率逐渐降低(P<0.05);细胞内GSH、GSH-Px和SOD含量显著降低(P<0.05),MDA、NF-κB蛋白表达量逐渐升高(P<0.05)。结论 n Cd S可能通过影响HKC内MDA、GSH、GSH-Px、SOD产生和NF-κB蛋白表达,从而导致HKC抗氧化系统的损伤而发挥氧化损伤作用。

[Objective]To explore the influence of nano-cadmium sulfide （nCdS） on oxidative damage of human kidney cell（HKC） and its mechanism.[Methods]The survival rates of HKC in logarithmic growth phase exposed to normal saline （control group） and different concentrations of nCdS （experimental group） were observed by MTT assay. The contents of MDA, GSH, GSH-Px and SOD were detected, and the expression status of NF-KB protein in cells was observed by western blotting. [Results]With the increasing of nCdS dose, the survival rates of HKC decreased （P〈0.05）. The content of GSH and vitalities of GSH-Px and SOD decreased （P〈0.05）, the contents of MDA and NF-κB protein expression increased （P〈0.05）.[Conclusion]The nCdS can induce antioxidant system damage of HKC by influencing the production of MDA, GSH, GSH-Px and SOD and expression of NF-κB protein to play a role of oxidative damage.