壳聚糖温敏性水凝胶蛋白载体的制备及其性能研究

Preparation and Properties of Chitosan Thermosensitive Hydrogels for Protein Drug Delivery

为了增强蛋白类药物的活性和寿命,应用物理交联技术制备了壳聚糖（CS）/京尼平（GP）/明胶（G）/β-甘油磷酸钠（β-GP）共混的温敏性水凝胶,采用红外光谱（FT-IR）、透射电镜（SEM）及降解实验对壳聚糖水凝胶的理化性能进行了表征;并研究了其对牛血清蛋白（BSA）的释放规律.结果显示：壳聚糖温敏性水凝胶的凝胶时间较短,约为50-126s;透射电镜显示4种物质相互交联为互通的多孔网络,结构致密均一;壳聚糖水凝胶具有一定的降解能力;BSA释放时间长达170h,最终释放率为76%-88%,蛋白释放曲线符合药物释放模型.

In order to enhance the activity and life-span of protein,which was applied a thermosensitive chitosan（CS）/genipin（GP）/genlatin（G）/β-glycerophosphate（β-GP）hydrogel was developed using physical crosslinking method.The physicochemical properties of chitosan hydrogel was confirmed by infrared spectroscopy（FT-IR）,transmission electron microscopy（SEM）and degradation test,its ability to release BSA.The results showed that the gel time of chitosan thermosensitive hydrogel is short,about 40—140s;The SEM revealed that the four substances crosslinked to porous network with interworking and dense homogeneous;Chitosan hydrogel has a certain ability to degrade;The BSA release time up to 170 hand the final release rate was 76%—88%,Protein release profiles are consistent with the model.