经血源子宫内膜干细胞复合3D打印PLGA支架体外培养的相容性研究

Cellular Compatibility of Menstrual Blood-derived Mesenchymal Stem Cells in Three-dimensional Printing PLGA Scaffolds

[目的]通过经血源子宫内膜干细胞(menstrual blood-derived mesenchymal stem cells,Men SCs)与3D打印PLGA支架材料的复合培养,探讨构建组织工程化骨软骨的可行性。[方法]预处理3D打印PLGA支架,取第5代Men SCs,倒置显微镜下观察细胞生物学特性,按1.0×106/m L的密度接种到PLGA支架材料上复合培养,通过倒置显微镜、Mico-CT、扫描电镜和组织病理学进行观察,并借此判断Men SCs与3D打印PLGA支架材料复合体外培养的融合性。[结果]Men SCs生长良好,细胞平铺生长,呈梭形或纺锤形,胞质薄,核圆居中,具有成纤维细胞形态。细胞传至第5代,为长梭形细胞单层,呈漩涡状排列。PLGA支架的微观呈现相互连通的多孔结构,结构疏松,孔隙大且互相交通,孔壁较薄。Men SCs在PLGA支架材料上生长状态良好,细胞在支架表面和内部生长,支架孔径内有细胞基质样组织连接,表明Men SCs与3D打印PLGA支架材料复合体外培养的融合性良好。[结论]经血源Men SCs是骨组织工程适宜的种子细胞,与3D打印的PLGA支架材料复合可体外构建组织工程骨。

[Objective] To investigate the feasibility of using three-dimensional（3D） printing PLGA loaded with menstrual blood-derived mesenchymal stem cells（Men SCs） as scaffolds for bone cartilage tissue engineering. [Methods] Three-dimensional printing PLGA was preprocessed. The five generation Men SCs were utilized. use invert microscope to observe cells biological property. According to 1.0 ×10^6/m L cells seeded onto PLGA scaffold composite culture, invert microscope, Mico-CT, scanning electron microscope and histopathology were observed.[Results] MSCs grew well, cells were plated to grow,fusiform or spindle row, thin cytoplasmic and nuclear circle centered, with fibroblast morphology. Cells to the fifth generation had long spindle cell monolayer and swirling arrangement. PLGA scaffolds exhibited microscopic interconnected porous structure, loose structure, large pores and mutual traffic,thinner hole wall. Men SCs on PLGA scaffold grew well, mainly in the scaffolds surface and inside growth, the scaffolds aperture had cell matrix organization connection.[Conclusion] Men SCs are ideal seeding cells for bone cartilage tissue engineering. And the PLGA scaffolds by three-dimensional printing with Men SCs may be in vitro construction as bone cartilage formation.