激光和LED光源对原卟啉Ⅸ光动力效应的基础研究

Effect of PpⅨ-PDT Irradiated by Laser and LED

目的观察激光光源和LED光源激发原卟啉Ⅸ(ProtoporphyrinⅨ,PpⅨ)产生的单线态氧产量,在溶液体系评价两种光源的对PpⅨ介导的光动力效应。方法利用单线态氧特异的商业化荧光探针定量检测激光和LED辐照PpⅨ产生的单线态氧产量,比较两种光源激发产生的单线态氧产量比值。结果在17.68 m W/cm2、35.37 m W/cm2、70.74 m W/cm2三档不同的功率密度下,激光与LED辐照PpⅨ产生的单线态氧比值均约等于1,两种光源相同功率密度条件下的单线态氧比值无显著统计学差异。结论在相同的功率密度下,635 nm激光和633 nm LED激光在溶液体系中经PpⅨ-PDT单线态氧产量比值是相等的,而细胞和生物组织中的PDT效应还有待于进一步探索。

Objective The purpose of this study is evaluate the effectiveness of different light source（ laser and LED） for PpⅨ-PDT by comparing the singlet oxygen quantum yields of PpⅨ irradiated by the laser and LED.Methods Singlet oxygen generated from illuminated PpⅨ were measured by using commercialized fluorescence probe and the singlet oxygen quantum yields ratio of these two light source were estimated.Results The singlet oxygen quantum yields ratios of laser and LED are approximately equal to 1 with different power densities of 17. 68 m W / cm2、35. 37 m W/cm2、70. 74 m W/cm2. No statistically significant difference were found between three sets of data.Conclusions The molecular-level photodynamic efficacy of PpⅨ-PDT with 635 nm laser was similar to that with 633 nm LED system.