不同条件下转cry1Ab基因玉米植株残体中杀虫蛋白降解动态

Degradation dynamics of Cry1Ab insecticidal protein within transgenic Bacillus thuringiensis corn plant debris under different conditions

为明确转cry1Ab基因玉米植株残体中Cry1Ab杀虫蛋白在不同条件下的降解动态,选取转cry1Ab基因玉米MON810和Bt11,采用ELISA方法测定4种条件下秸秆中Cry1Ab杀虫蛋白的残留量。结果表明,在不同条件、不同取样时间,2种玉米秸秆中Cry1Ab杀虫蛋白的残留量差异显著;整株秸秆中的Cry1Ab杀虫蛋白降解速度比粉碎后的慢;秸秆粉碎埋入土壤后播种冬小麦,玉米秸秆中Cry1Ab杀虫蛋白降解速度最快,且可完全降解;在不同条件下,MON810玉米秸秆中Cry1Ab杀虫蛋白的降解速度比Bt11玉米慢,MON810和Bt11玉米秸秆中Cry1Ab杀虫蛋白的DT50分别为10.2~207.8、13.6~124.0 d,DT90分别为185.1~368.3、45.2~224.0 d。研究表明,在不同条件下MON810和Bt11玉米秸秆中Cry1Ab杀虫蛋白的降解速度不同,依次为秸秆粉碎后埋在土壤后种植冬小麦〉秸秆粉碎埋在玉米田土壤中〉秸秆粉碎后放在地表〉整株秸秆放在地表。

In order to clarify the degradation dynamics of Cry1 Ab insecticidal protein in the plant debris of cry1 Ab transgenic corn under different conditions,the contents of Cry1 Ab protein in the cry1 Ab transgenic corn MON810 and Bt11 were quantified by using enzyme-linked immunosorbent essay（ ELISA） method. The results showed that there were significant differences in the degradation rates of Cry1 Ab insecticidal protein of MON810 and Bt11 under different conditions and sampling times. The degradation rate of Cry1 Ab insecticidal protein in the whole plant was slower than that in the smashed plant debris. The degradation rate of the Cry1 Ab insecticidal protein in the smashed stalks buried in the winter wheat field was faster than the other treatments. The degradation rate of the Cry1 Ab insecticidal protein in MON810 was slower than that of Bt11. The DT50 of the Cry1 Ab insecticidal protein in MON810 and Bt11 were 10. 2- 207. 8,13. 6- 124. 0 d,and the DT90 values were 185. 1- 368. 3,45. 2- 224. 0d,respectively. The degradation rates of Cry1 Ab insecticidal protein were different under different conditions. In four treatments,the degradation rate of Cry1 Ab insecticidal protein in the smashed stalksburied in winter wheat field was the highest,followed by the smashed stalks buried in after-harvest corn field,in the smashed stalks placed on the surface of the soil,and in whole corn plants on the ground.