植物乳杆菌L01对人结肠癌细胞系Caco-2的黏附性能

Study on the adhesive properties of Lactobacillus plantarum L01 upon human colon cancer cell line Caco-2

了解植物乳杆菌L01黏附性能,为其在功能食品中的应用提供理论基础。采用体外黏附试验法对植物乳杆菌L01黏附于人结肠癌细胞系Caco-2性能进行研究。考察了菌体浓度、生长阶段、作用时间、环境p H值、单糖种类和金属离子对植物乳杆菌L01黏附性能的影响。试验结果表明:共培养2 h后菌体浓度达到1×10~8CFU/m L,菌体数趋于饱和,稳定期的植物乳杆菌L01对Caco-2细胞的黏附效果最好;环境p H值对于黏附作用影响显著(P<0.01),在弱酸范围黏附性最强;4种单糖(D-葡萄糖、D-半乳糖、D-甘露糖、甲基-α-D-甘露糖苷)对植物乳杆菌L01黏附抑制作用中,甲基-α-D-甘露糖苷、D-甘露糖均有显著抑制(P<0.01)作用,另外两种单糖D-葡萄糖、D-半乳糖无显著变化(P>0.05);金属离子Ca^(2+)、Mg^(2+)对植物乳杆菌L01黏附作用影响不显著(P>0.05)。由此得出结论,植物乳杆菌L01对Caco-2细胞的黏附特性受多种外部因素的共同影响。

Studying the adhesive properties of Lactobacillus plantarum L01,which providing theoretical basis for its application in functional food. Adhesive test was adopted to study the adhesive ability of Lactobacillus plantarum L01 upon colon cancer cell line Caco-2 in vitro. The effects of various factors on the adhesive properties were studied,including cell concentration,growth stage,time,p H,kinds of monosaccharide and metal ion. The results showed that co-cultivation after 2 hours,cell concentration was 1 × 10~8 CFU / m L and reached saturation,the adhesive effect of Lactobacillus plantarum L01 in stationary phase was the best; the p H of the solution demonstrated effect significantly（ P〈0. 01） on the adhesive ability,and which became strongest at weak acid. Around the four monosaccharides,the adhesive ability was decreased significantly（ P〈0. 01） with the addition of D-mannose and methyl-α-D-mannopyranoside,whereas it had no significantly effects（ P〉0. 05） on the adhesive ability when the D-glucose and the D-galactose were added; Ca^2＋,Mg^2＋had no significant effects（ P〉0. 05） on the adhesive ability of Lactobacillus plantarum L01 either. In conclusion,the adhesive properties of Lactobacillus plantarum L01 upon Caco-2 cells were affected by various factors.