生、麸炒苍术对大鼠AQP1、AQP5及血液流变学的影响

Effects of Crude Atractylodis Rhizoma and Processed Atractylodis Rhizoma on AQP1,AQP5 and Hemorheology in Healthy Rats

目的:通过比较苍术麸炒前后对健康大鼠影响的差异,证明生苍术燥性明显而麸炒后燥性缓和的传统炮制观点。方法:以健康大鼠为实验对象,分别给予不同剂量的生苍术、麸炒苍术、阿托品以及蒸馏水,并用ELISA法测量不同组别大鼠给药后颌下腺水通道蛋白1(AQP1)、水通道蛋白5(AQP5)的含量,测定颌下腺指数、血液流变学指标、肠管含水量等生理指标。结果:灌服生苍术提取液的大鼠与灌服麸炒苍术提取液的大鼠以及空白对照组大鼠的颌下腺指数、血液流变学等指标存在显著差异,生苍术组的肠管含水量显著低于其他组。结论:初步证明调控肠管含水量、颌下腺指数以及血液流变性是苍术体现"燥性"的主要作用机理,且麸炒使苍术的燥性得以缓和。

Objective： To study the different effects of crude Atractylodis Rhizoma and processed Atractylodis Rhizoma in healthy rats,in order to prove the traditional theory that the crude Atractylodis Rhizoma has dry effects and the dry effects can be weaken by processing. Methods： Health rats had been orally administered with pure water,crude Atractylodis Rhizoma,processed Atractylodis Rhizoma and atropine. The concentration of AQP1 and AQP5 in submaxillary gland were measured by ELISA. Their index of submaxillary gland,hemorheology and moisture content of intestine were also measured. Results： There were obvious differences of concentration of AQP1 and AQP5 in submaxillary gland,index of submaxillary gland,hemorheology and moisture content of intestine between the rats which had been orally administered crude Atractylodis Rhizoma and the rats administered processed Rhizoma Atractylodes. Conclusions：The dry effects of Atractylodis Rhizoma works on rats＇ moisture content of intestine,index of submaxillary gland and hemorheology. The dry effects can be weaken by processing.