库拉索芦荟凝胶多糖的GPC指纹图谱研究

GPC Fingerprint Chromatograms of Aloe vera Leaf Gel Polysaccharides

目的:建立同一产地(北京)和不同产地库拉索芦荟凝胶多糖的凝胶渗透色谱(GPC)指纹图谱,并将其用于市售芦荟产品的质量评价及常见掺假物检测。方法:库拉索芦荟凝胶多糖采用水提醇沉法制备,GPC分析采用Shodex SUGAR KS-805色谱柱(300 mm×8.0 mm,7μm)和Shodex SUGAR KS-803色谱柱(300 mm×8.0 mm,6μm)串联,以0.1 mol/L NaNO_3溶液(含0.2‰Na N3)洗脱,流速0.8 m L/min,柱温60℃,示差折光检测器(RID)检测温度50℃。结果:建立了库拉索芦荟凝胶多糖GPC指纹图谱的共有模式,标定4个共有峰,10批次同一产地(北京)和10个不同产地样品的相似度均高于0.9。用指纹图谱判断市售的3种芦荟产品均含有芦荟凝胶多糖,但制作工艺不同,并有效鉴别出了几种常见掺假物。结论:该文建立的方法简单、准确、重复性好,为库拉索芦荟凝胶产品的真伪鉴别和品质评价提供了依据。

Objective： To establish the gel permeation chromatography（ GPC） fingerprint chromatograms of polysaccharides in Aloe vera leaf gel from the same habitat（ Beijing） and different habitats for evaluating the quality of Aloe vera leaf gel products commercially available and testing common adulterated substances.Methods： The samples were prepared by water-extraction and alcohol-precipitation method.GPC separation was performed on a Shodex SUGAR KS-805（300 mm × 8.0 mm,7 μm） column and a Shodex SUGAR KS-803（ 300 mm × 8.0 mm,6 μm） column at the temperature of 60 ℃ by eluting with 0.1 mol/L NaNO^3（ containing 0.2‰ Na N3） at a flow rate of 0.8 m L / min,the chromatographic effluent was detected by refractive index detector（ RID） at the temperature of 50 ℃.Results：The common pattern of GPC fingerprint chromatograms was established and four common peaks were demarcated.The similarities of samples from the same habitat（ Beijing） and different habitats were over 0.9.Taking the GPC fingerprint chromatograms for the qualified model,three commercially available aloe products were evaluated to be made of Aloe vera by the different manufacturing processes and four common adulterated substances of aloe polysaccharides were identified effectively.Conclusion： The method is simple and accurate with a good reproducibility,and it can be used for the identification and quality evaluation of Aloe vera leaf gel products.