复方总黄酮对小鼠慢性酒精性睾丸损伤的作用研究

Protective effects of compound flavones against alcohol-induced testicular injury in mice

目的探讨复方总黄酮对慢性酒精睾丸损伤小鼠的保护作用。方法 100只SPF级C57BL/6小鼠随机分为5组:正常对照组、慢性酒精组、酒精+低剂量药物组、酒精+高剂量药物组、药物对照组,每组20只。采用酒精浓度递增梯度法建立小鼠慢性酒精睾丸损伤模型,同时用复方总黄酮进行干预治疗,连续6个月,测定睾丸组织中超氧化物歧化酶(SOD)、丙二醛(MDA)以及睾酮含量。HE染色观察组织形态学变化,电镜观察其超微结构改变。结果慢性酒精组小鼠睾丸组织中MDA含量明显升高,SOD活力以及睾酮含量下降,与正常对照组比较差异有显著性(P<0.05);此外,睾丸生精上皮结构破坏,支持细胞和各级生精细胞均有退化变性,复方总黄酮可呈剂量依赖性地逆转上述改变。结论复方总黄酮对慢性酒精睾丸损伤小鼠的保护作用可能与其抑制脂质过氧化和提高睾酮活性密切相关。

Aim To study the protective effect of the compound flavones on chronic alcohol induced testicular damage in mice.Methods One hundred SPF C57BL/6 mice were randomly divided into 5 groups:normal control group,chronic alcohol group,alcohol+low-dose drug group,alcohol+high dose drug group,and drug control group.Chronic alcohol testicular injury model in mice was established by intragastric administration of increasing dose of alcohol every four weeks for 6 months,meanwhile compound flavone intervention was in process.The activity of super oxide dismutase(SOD),the levels of malondialdehyde(MDA)and testosterone in testicular tissue were measured.HE staining was used to observe testicular histomorphology,and ultrastructure changes were detected by electron microscope.Results In chronic alcohol group,MDA content was obviously increased,while SOD and testosterone levels were decreased compared with normal control group(P<0.05).In addition,germinal epithelium,support cells and sperm production at all levels showed degraded degeneration in chronic alcohol group.However,compound flavonoids could successfully reverse alcohol-induced testicular damage in a dose-dependent way.Conclusion The compound of flavones may have therapeutic potential for alcohol-induced testicular injury through inhibiting lipid peroxidation and increasing the level of testosterone.