烟粉虱MED隐种对13种植物挥发性物质的行为反应

Behavioral responses of Bemisia tabaci MED to 13 plant volatiles

为明确烟粉虱Bemisia tabaci MED隐种对不同植物挥发性物质的行为选择,采用Y型嗅觉仪测定了烟粉虱雌、雄成虫对13种植物挥发性物质的趋向行为反应。结果显示,烟粉虱MED隐种雌、雄成虫对不同浓度植物挥发性物质的选择性存在明显差异。1、0.01和0.0001μL/μL浓度的芳樟醇、反-2-己烯醛和桉树脑对烟粉虱MED隐种雌、雄虫校正反应率均为正值,具显著吸引效果;而p-伞花烃、月桂烯、3-蒈烯、α-蒎烯和顺-3-己烯-1-醇对雌、雄成虫的校正反应率均为负值,说明其对烟粉虱MED隐种雌、雄虫具有不同程度的趋避性。雌烟粉虱对0.0001μL/μL浓度桉树脑的选择率达最大值70.30%,与对照差异显著,具显著吸引作用。雌、雄烟粉虱对0.01μL/μL浓度的顺-3-己烯-1-醇的选择率最小,仅为24.75%和27.03%,具显著驱避作用。因此,烟粉虱MED隐种雌、雄成虫对不同浓度植物挥发性物质趋性反应不同,可能是烟粉虱环境适应性的表现。

In order to evaluate the reactive preference of Bemisia tabaci MED to the volatiles from various plants,the behavioral response of whitefly adults to volatile odors were observed via a Y-type olfactometer,which was conducted to investigate the repellent effect of 13 volatile odors to B. tabaci. The results showed that there was significant difference in the orientation rate to different concentrations of aliphatic compounds between the male and female. The response rates of both B. tabaci females and males were positive to 1,0. 01 and 0. 0001 μL / μL of linalool,trans-2-hexenal,and 1,8-cineole,which showed significant attractive effect. However,the response rates were negative value in all concentrations of p-cymene, myrcene,3-carene, α-pinene and cis-3-hexen-1-ol, which indicated that they were repellent to both B. tabaci females and males. It was found that 0. 0001 μL / μL 1,8-cineole had the highest attraction to B. tabaci MED females with the selectivity of 70. 30%. B. tabaci MED females and males had the lowest selectivity of 24. 75% and 27. 03% to 0. 01 μL / μL cis-3-hexen-1-ol,respectively.B. tabaci MED males and females had different orientational behaviors to different concentrations of plant volatiles,which might reflect their environmental adaptiveness.