气相色谱-质谱结合化学计量学分析莪术挥发油成分（英文）

Analysis of Essential Oil Composition of Commercial Curcumae Rhizoma from Different Processed Products and Sources by GC-MS coupled with Chemometrics

中药市场上的莪术来自不同的官方品种和炮制品种,因而存在着一定的混用情况。为了初步比较研究市售莪术的化学成分,我们对其主要的活性成分莪术挥发油进行了研究。采用气相色谱-质谱结合化学计量学方法对来自于16个批次的不同官方品种和炮制品种的挥发油进行了化学分析和MCF-7细胞毒性检测。鉴定出53个化学成分,其中包括11个首次鉴定出的化合物。结果表明16批样品显示相似的抗肿瘤活性,而其化学成分在种间存在显著差异,而炮制品间未见差异。因此,来自于Curcuma kwangsiensis和C.phaeocaulis的挥发油应该与C.wenyujin区分开来。三个官方品种应当被进行深入的比较研究和区别应用。

The three official species and processed products of Chinese medicine Curcumae Rhizoma were commonly mixed used. In order to preliminary study and compare the chemical components of Curcumae Rhizoma commercially available,the essential oil,which was reported to be the main active components,was taken as the research object in this study. The essential oil composition from different processed products and sources of Curcumae Rhizoma was analyzed by GC-MS coupled with chemometrics. In addition,their cytotoxic activity on MCF-7 cell lines was also assessed. As a result,53 components,including 11 components for the first time,were identified from Curcumae Rhizoma volatile oil. In the comparative study of species,although their cytotoxic activity on MCF-7 cell lines were equally effective,significant differences were found intergroup by SPSS analysis; and the comparative study of processed products showed no significant difference. The essential oils from Curcuma kwangsiensis and C. phaeocaulis should be distinguished from C. wenyujin,the official source of essential oil of Curcumae Rhizoma.