不同品种葡萄干中糖类成分分析

Extraction and Analysis of Saccharides in Different Varieties of Raisin

采用衍生化后GC-MS和HPLC-ELSD对新疆产8个品种葡萄干中糖类成分进行定性和定量分析。通过单因素实验考察提取温度、料液比、提取溶剂对糖类成分提取的影响。结果显示：经比对标准品在相同衍生化和GC-MS条件下的保留时间,8种葡萄干中共检出8种糖（果糖、葡萄糖、甘露糖、蔗糖、肌糖、麦芽糖、半乳糖、D-山梨糖醇）。在乙醇浓度50%,料液质量比1∶8,温度80℃的提取条件下葡萄干中糖的提取率最高。建立了对葡萄干中含量较高的果糖、葡萄糖、蔗糖进行定量检测的HPLC-ELSD方法,方法线性良好（R^2〉0.9992）,精密度（RSD）〈2.0%,回收率92%~109%。8种葡萄干中除索索外,总含糖量均大于690 mg/g,果糖和葡萄糖在各品种葡萄干的糖含量中均占主导地位。

The saccharides in 8 different varieties of raisin were qualitatively analyzed with GC-MS,and quantitatively analyzed by HPLC-ELSD using Prevail Carbohydrate ES column under gradient elution with water and acetonitrile. The parameters of extraction temperature,solvent and solid / liquid ratio on the extraction of saccharides in raisin were investigated with single factor test. Results showed that optimized parameters for extraction were that the concentration of ethanol was 50%（ V / V）,the extraction temperature was 80 ℃,and solid / liquid ratio was 1∶ 8. Eight saccharides（ fructose, glucose, mannose, sucrose, inositol,maltose,galactose,D-sorbitol） were detected by GC-MS. The contents of fructose,glucose and sucrose in different raisins were quantitatively analyzed by HPLC-ELSD. The calibration curves exhibited good linearity（ R2〉 0. 9992）,the recoveries ranged from 92% to 109%,and the relative standard deviation（ RSD） was lower than 2%. The total content of saccharides in varieties of raisins,except for Black corinth,were greater than 690 mg / g. The major ingredient was fructose and glucose.