香椿子不同极性部位对糖尿病周围神经病变的保护作用

Protective Effect of Toona sinensis Seed Extracts on Diabetic Peripheral Neuropathy

为考察香椿子对糖尿病周围神经病变(DPN)的保护作用,采用系统溶剂法分离香椿子醇提物的不同极性部位,分别考察体外抗氧化活性,以DPN大鼠为模型,探讨各部位对DPN相关指标的影响。结果表明香椿子提取物具有良好的抗氧化活性,可抑制DPPH自由基和抑制肝脂质过氧化,能够改善试验大鼠一般状况,降低血糖,恢复坐骨神经神经传导速度和周围神经痛觉敏感性,上调血清NGF-β水平,缓解神经组织中脂质过氧化程度和恢复抗氧化酶活力。试验表明,香椿子可通过干预氧化应激和上调NGF等机理起到保护DNP的作用,综合评价,正丁醇部位效果最佳。

The aim of this study was to clarify whether Toona sinensis seed（ TS） extracts were therapeutic agents against diabetic peripheral neuropathy（ DPN）. Four polar fractions were separated from TS ethanol extracts,after that their antioxidant activities were investigated in vitro. Male Wistar rats were used to induce DPN model and DPN related indicators were detected. The results showed that the extracts exhibited favorable antioxidant activity against DPPH radical and lipid peroxidation. After treating with TS extracts,general features of DPN rats were improved,the blood glucose levels and MDA contents in sciatic nerve were decreased,the serum NGF-β contents,SOD and GSH-PX activities were increased and the sciatic nerve conduction velocity was recovered significantly（ P 0. 05）. This study indicated that TS extracts can alleviate DPN through inhibiting oxidative stress and up-regulating NGF-β and among the four extracts,the n-butyl alcohol extract showed the best effect.