汉坦病毒与宿主线粒体D-loop和cyt b基因共进化分析

Co-divergence of hantavirus with its hosts' mitochondrial D-loop and Cyt-b sequences

目的比较汉坦病毒(HV)抗原阳性和阴性的宿主动物线粒体D环区(D-loop)和细胞色素b(Cyt b)基因序列变化及与HV共进化的关系。方法采用直接免疫荧光法检测715份宿主动物鼠肺HV感染情况,提取HV抗原阳性和阴性黑线姬鼠鼠肺组织总DNA,应用聚合酶链反应(PCR)扩增宿主动物线粒体的D-loop和Cyt b基因,PCR产物纯化后测定序列,应用Clustal X生物学软件进行多重序列同源性分析,M ega软件绘制系统发生树进行分析比较。结果 HV抗原检测阳性和阴性鼠的遗传距离较小,阳性和阴性鼠的D-loop和Cyt b基因均有分布在同一个分支上。结论 HV感染与否与宿主线粒体基因无直接联系。

Objective To analyze the co-divergence of hantaviruses（ HV） with mitochondrial D-loop and cytochrome b（ Cyt-b） gene of its rodent hosts. Methods The sequences of mitochondrial D-loop and Cyt-b were adopted in the analysis. Total DNA was extracted from HV positive and negative lung tissue samples from Apodemus agrarius. The sequences of the D-loop and Cyt-b were amplified with PCR. The sequence similarity was assessed with Clustal X and the phylogenetic tree was estimated with MEGA using the maximum likelihood method. Results According to the phylogenetic tree,the study indicated that the HV positive and negative Apodemus agrarius were in same cluster. Conclusion There is no obvious relationship between the sequences of mitochondria of the hosts and the infection of hantavirus.