喷砂酸蚀对超细晶纯钛表面MC3T3-E1细胞粘附与增殖的影响

Biocompatibility of Ultrafine-grained Titanium Used for Implant after Sandblasting and Large-grits Acid-etching

目的:研究喷砂酸蚀对超细晶纯钛表面MC3T3-E1细胞粘附与增殖的影响。方法:将超细晶纯钛棒和纯钛棒切割为直径6mm,厚度3mm钛片试件,试验组为喷砂酸蚀超细晶纯钛组,对照组分别为未处理的超细晶纯钛组和喷砂酸蚀纯钛组。在对其表面形貌特征和亲水性进行检测后,在试件表面接种MC3T3-E1细胞,观察细胞的初期粘附情况,测定细胞密度,存活和生长状态。结果:喷砂酸蚀超细晶纯钛后,其表面形成大量微小的弹坑状凹陷,且表面具有良好的亲水性。接种细胞后,喷砂酸蚀超细晶纯钛初期粘附优于对照组;细胞密度在培养中后期优于对照组;而细胞活性在培养中期优于两对照组,培养后期3组间无明显差异。结论:喷砂酸蚀超细晶纯钛的表面性能得到改善,能诱导MC3T3-E1细胞在其表面粘附和增殖,可作为纯钛种植体种植体的替代材料。

Objective： To study the effect of sandblasting and large--grits acid--etching （SLA） on the adhesion and proliferation of MC3T3--E1 cells on ultrafine--grained titanium （UFG Ti）. Methods： Ti cylindrical specimen with 6mm in diameter and 3mm in height were incised from the bars of UFG Ti and Pure Ti. Experimental group （SLA--UFG Ti） was UFG Ti through SLA, while control group were UFG Ti through mechanical processing without any advanced processing （M--UFG Ti） and Pure Ti through SLA （SLA--Ti）. The surface morphology and hydrophily of the surfaces were analyzed. The MC3T3--E1 cells were seeded on the surfaces of titanium speci- mens. Cell initial adhesion, ceil density, and cell viability were observed. Results： Plenty of tiny sandpits with ir- regular shape were formed on the surface of SLA--UPG Ti group. Hydrophily of UFG Ti was improved after Equal --Channel Angular Pressing processing （ECAP）. At the early stage, cell adhesion on the surface of SLA--UFG Ti was better than that of control group, while cell density was more intensive in the middle stage and the later stage, Cell viability was better at the middle stage, while no significant difference at the later stage. Conclusion： The sur- face property of SLA--UFG Ti was improved. SLA--UFG Ti could induce adhesion and proliferation of MC3T3-- E1 cells. It might be a prospective substitute to pure Ti implant.