摘要
[目的]观察热疗联合阿霉素对人多发性骨髓瘤细胞株ARH-77的体外增殖抑制、凋亡及Bcl-2表达的影响。[方法]采用MTT法确定阿霉素的工作浓度,以该浓度进行化疗或与热疗的联合,选择温度40℃、41℃及42℃,作用于ARH-77细胞。采用绘制肿瘤细胞生长曲线、MTT法检测对肿瘤细胞增殖的抑制作用;流式细胞仪检测肿瘤细胞凋亡及Bcl-2表达。[结果]阿霉素IC50为8.16μg/ml,以此为实验的工作浓度。单纯热疗组60min对ARH-77细胞有抑制作用(P<0.01),并随温度增高而增强。单纯阿霉素化疗组对ARH-77细胞也有抑制作用。各热化疗组对ARH-77均有明显的抑制作用(P<0.01)。热疗组、化疗组及热化疗组的细胞凋亡率均较对照组显著升高,各组之间差异有统计学意义(P<0.01);Bcl-2蛋白表达下降,各组之间也有显著性差异(P<0.01)。[结论]热疗联合阿霉素能增强对ARH-77细胞的体外抑制作用,提高肿瘤细胞的凋亡率,下调Bcl-2蛋白的表达,为热化疗的临床应用提供实验室依据。
[Purpose] To investigate the influence of hyperthermia combined with adriamycin on the inhibition of proliferation,apoptosis and Bcl-2 expression in multiple myeloma cell line ARH-77. [Methods] The working concentration of adriamycin against ARH-77 was determined by MTT assay. The inhibition effect was evaluated by the growth curve of tumor cells and MTT assay. The apoptosis rate and Bcl-2 expression in ARH-77 were determined by flow cytometric analysis. [Results] The IC50 of adriamycin in the experiment was 8.16μg/ml,which was defined as its working concentration. Each hyperthermia group for 60 min showed obvious inhibition to ARH-77（P〈0.01）,which increased with the increasing of temperature. The adriamycin chemotherapy group inhibited the growth of ARH-77 too（P〈0.01）. Each thermochemotherapy group showed obvious inhibition effect to ARH-77（P〈0.01）.Compared with control group,cell apoptosis rates increased and Bcl-2 expression decreased significanty in hyperthermia group,chemotherapy group and thermochemotherapy group,with significant difference among the groups（P〈0.01）. [Conclusion] Hyperthermia combined with adriamycin chemotherapy might promote the inhibition effect to ARH-77,increase the apoptosis rate and down-regulate Bcl-2 expression,which provide laboratory evidence for the clinical application of thermochemotherapy.
出处
《中国肿瘤》
CAS
2016年第2期138-142,共5页
China Cancer
基金
CSCO-先声抗肿瘤血管靶向治疗科研基金(Y-S2014-016)
