新疆哈萨克族与汉族食管鳞癌患者血浆中microRNA-31的表达及其意义

Expression and signification of micro RNA-31 in the plasma of esophageal squamous cell carcinoma of Xinjiang Kazak and Han nationality patients

目的:检测新疆哈萨克族及汉族食管鳞癌(esophageal squamous cell carcinoma,ESCC)患者血浆中microRNA-31(miR-31)表达情况,分析miR-31与食管鳞癌的相关关系。方法:收集两民族食管鳞癌患者及健康受检者血浆标本各20例,应用实时荧光定量PCR技术检测血浆中miR-31的相对表达。结果:食管鳞癌组血浆中miR-31的表达均高于其正常对照组,且哈萨克族食管鳞癌患者血浆中miR-31的表达与肿瘤分化程度有关联(P<0.01);哈萨克族食管鳞癌组和正常对照组血浆中miR-31的相对表达分别高于汉族食管鳞癌组(P=0.008)和正常对照组(P=0.027)。结论:miR-31可能参与了哈萨克族、汉族食管鳞癌的发生、发展。miR-31可能是哈萨克族较汉族食管鳞癌高发的又一致病因素。

Objective To analyze the correlation between miR-31 and ESCC in expression of miR-31 in the plasma of ESCC in Xinjiang Kazak and Han nationality patients.Methods The plasma samples were collected respectively from patients with ESCC in 20 cases and healthy subjects in 20 cases.The relatively expression of miR-31 was detected by real-time Q-PCR.Results The expression of miR-31 with ESCC were higher than those of the normal control group,which related to the degree of tumor differentiation in Kazak ESCC patients（P 〈0.01）;the levels of miR-31 relative expression in Kazak were higher than that of Han（P = 0.008,P = 0.027）.Conclusion miR-31 may be involved in the occurrence of ESCC in Han and Kazak nationality.miR-31 might be another risk factor in high incidence of ESCC in Kazak than Han nationality.