不同地区牦牛肉中FABP基因Real-time PCR表达差异分析

Analysis of FABP gene's expression difference in yak meat from different regions by Real-time PCR

为了研究不同地区牦牛肉品质的差异,本实验从大通、九龙、河南、迪庆、红原五个地区采集4岁公牦牛肌肉样品,提取总RNA,在逆转录聚合酶的作用下生成c DNA,以逆转录产物为模板,利用Real-time PCR检测牦牛肌肉中脂肪酸结合蛋白基因的表达水平。将迪庆地区牦牛作为对照组,肌肉中脂肪酸结合蛋白基因的c DNA实时荧光定量的2-ΔΔCt值设定为1,则大通、九龙、河南、红原地区的牦牛肌肉中脂肪酸结合蛋白基因的c DNA实时荧光定量的2-ΔΔCt值分别为4.18、2.94、9.95、10.73,从而得出不同地区牦牛肌肉中脂肪酸结合蛋白基因的相对表达水平,在分子水平上更加准确地评估不同地区牦牛肉品质。实验结果表明,红原和河南地区的牦牛肉品质最优,而迪庆地区牦牛肉品质较差。

In order to research the difference of yak meat＇s quality in different regions,total RNA was extracted from 4-year-old male yak ＇s muscle samples of Datong,Jiulong,Henan,Diqing,Hongyuan,and then c DNA was generated under the action of reverse transcription polymerase,at last,the production of reserve transcription reaction was utilized to carry out RT-PCR and detect the expression levels of FABP gene. The results were that if the yak muscle sample of Diqing was taken as the control group and the 2^（-ΔΔCt）value of it was set to 1,the fluorescence quantitative 2^（-ΔΔCt）value of Datong,Jiulong,Henan,Hongyuan yak muscles were 4.18,2.94,9.95,10.73 respectively. So that the relative expression quantity of FABP gene in yak muscles could be obtained to evaluate yak meat＇s quality in different regions on molecular level. The yak meat＇s quality of Hongyuan and Henan was the best while that of Diqing was the worst.