河蚬肉抗氧化肽的分离及活性研究

Separation and activity assay of antioxidant peptides from Corbicula fluminea meat

目的:为制备具有抗氧化活性的河蚬肉抗氧化肽。方法:河蚬肉酶解液经膜分离后,采用Sephacryl S-100 HR凝胶层析柱进行分离,得到抗氧化性最强的组分经CM Sepharose FF离子交换层析柱进一步分离,并对各组分的体外抗氧化活性进行测定。结果:经Sephacryl S-100 HR凝胶层析柱分离得到5个活性组分A、B、C、D和E,其中组分C的抗氧化活性较强,其总抗氧化能力A_(700 nm)、羟自由基清除率和DPPH自由基清除率分别为1.79、83.33%和26.35%。组分C经CM Sepharose FF离子交换层析柱进一步分离得到2个活性组分C1和C2,其中C2的抗氧化活性最强,总抗氧化能力A_(700 nm)、羟自由基清除率和DPPH自由基清除率分别为2.03、92.34%和35.94%。结论:河蚬酶解液抗氧化肽的羟自由基清除能力突出,该研究为河蚬肉抗氧化肽的开发提供理论技术依据。

Objective：In order to separate the antioxidant peptides from Corbicula fluminea meat. Methods：In this experiment,enzymatic hydrolysates of Corbicula fluminea meat were isolated by Sephacryl S-100 HR gel chromatography after membrane separation,the oxidation resistance of the strongest components was further purified by CM Sepharose FF ion exchange chromatography and antioxidant activity in vitro of each group was measured. Results：The result showed that the enzymatic hydrolysates of Corbicula fluminea meat was fractionated into five active fractions by ephacryl S-100 HR gel chromatography,named as A,B,C,D and E.Among these,the fraction C showed the higher antioxidant activity,the total antioxidant capacity,scavenging ability of hydroxyl and DPPH radical were 1.79,83.33%,26.35%,respectively. Component C was further separated into two active components by CM Sepharose FF ion exchange chromatography,named as C1 and C2,the fraction C2 showed the highest antioxidant activity,the total antioxidant capacity,scavenging ability of hydroxyl and DPPH radical were 2.03,92.34%,35.94%,respectively. Conclusions ：The radical scavenging ability of Corbicula fluminea meat was outstanding,which provides a theoretical basis for the deep processing of the peptide.