固载ClO_2保鲜剂对米良猕猴桃腐败菌的抑菌活性

Antimicrobial activity of solid ClO_2 preservative on miliang kiwifruit pathogenic fungi

为考察固载Cl O2保鲜剂对米良猕猴桃腐败菌的抑菌效果,通过PDA平板培养法从米良猕猴桃病害组织中分离腐败菌,运用有伤和无伤接种法筛选最强致病力菌株,经形态学及分子生物学鉴定菌株,采用菌丝生长速率测定法考察固载Cl O_2保鲜剂对最强致病力菌株的抑菌效果。结果显示,从米良猕猴桃病害组织中分离出的7种腐败菌中,有3种致病力最强的菌株,经鉴定分别为漆斑菌属(Myrothecium sp.)、茎点菌属(Phoma sp.)和交链孢霉属(Alternaria sp.);常温下固载Cl O_2保鲜剂对3株菌株均有抑制作用,但都不能完全抑制菌种的生长,抑制活性交链孢霉属>漆斑菌属>茎点菌属;2℃下固载Cl O_2保鲜剂几乎能使3株菌株完全受到抑制。结果表明,固载Cl O_2对米良猕猴桃腐败菌有较好的抑菌效果,可以作为米良猕猴桃的保鲜剂用于降低腐败率。

In order to investigated antimicrobial activity of solid chlorine dioxide preservative on miliang kiwifruit,pathogenic fungi were isolated from diseased tissue by PDA plate. Using injury and no injury inoculation to screening strongest virulence strains. Morphological and molecular way were used to identify strains. The antimicrobial activity of solid ClO_2 preservative on the most virulent strains was investigated by using the method of mycelium growth rate. Research had revealed that seven pathogen strains were parted from rotten miliang kiwifruit. Three of them were deemed as the most virulent strains and identified as Myrothecium sp.,Phoma sp.and Alternaria sp. All three strains were inhibited by solid ClO_2 preservative under room temperature but couldn＇t completely inhibit their growth. The sequence of inhibition was Alternaria sp.,Myrothecium sp. and Phoma sp. The three strains were almost inhibited by solid ClO_2 preservative under 2 ℃. Solid ClO_2 spoilage of miliang kiwifruit had a good antibacterial effect. It can be used as a preservative for reducing the rate of corruption.