摘要
目的建立姜炭药材的超高效液相色谱-二极管阵列检测器(ultra performance liquid chromatography-photodiode array,UPLC-PDA)指纹图谱。方法采用UPLC BEH色谱柱C18(2.1mm×100mm,1.7μm),流动相为水-乙腈,梯度洗脱;流速0.25mL/min;柱温30℃;检测波长为280nm;分别对14批样品指纹图谱进行相似度分析、聚类分析和偏最小二乘法判别分析。结果实验指认了指纹图谱15个共有色谱峰中的5个。14批不同产地姜炭药材中9批样品相似度大于0.9,可聚为4大类,其中10个色谱峰的VIP得分值大于1.0。结论所建立的指纹图谱方法简便、稳定、可行,可用于姜炭的质量评价及分类。
Objective To establish the fingerprint of ginger charcoal by ultra-performance liquid chromatography-photodiode array （UPLC-PDA）. Methods The UPLC BEH column Cla （2.1 mm × 100 mm, 1.7 μm） was used with a mobile phase of water-acetonitrile for gradient elution at a flow rate of 0.25 mL/min, a col- umn temperature of 30 ℃, and a detection wavelength of 280 nm; similarity analysis, cluster analysis, and partial least squares discriminant analysis were performed for the fingerprints of 14 batches of samples. Results The laboratory test identified 5 out of 15 common chromatographic peaks in the fingerprints; of all the 14 batches of ginger charcoal produced in different places, 9 had a similarity higher than 0.9 and were clustered into 4 categories, and 10 chromatographic p tablish fingerprints is simple, stable, rization of ginger charcoal.
出处
《安徽中医药大学学报》
2016年第1期86-89,共4页
Journal of Anhui University of Chinese Medicine
基金
国家自然科学基金项目(81102811)
安徽省自然科学基金项目(10040606Q40)
国家中医药重点学科临床中药学建设项目(国中医药人教发[2012]32号)
关键词
姜炭
超高效液相色谱指纹图谱
偏最小二乘法判别分析模式识别
Ginger charcoal
UPLC ognition eaks had a VIP score higher than 1. O. Conclusion The method to es and feasible, and can be applied for the quality evaluation and catego fingerprint
Partial least squares discriminant analysis for pattern rec
