比较两种方法对牛鲍氏计数板中细胞计数结果的影响

Comparison of influence of two methods on cell count results in Neubauer counting chamber

目的比较牛鲍氏计数板的传统计数方法和新计数方法对细胞计数结果的影响。方法用传统计数方法和新计数方法计数118份抗凝血标本中的红细胞,然后将结果与标准值对照。结果在109份有效样本中,有57份(52.29%)用新方法计数结果更准确,40份(36.70%)用传统方法计数结果更准确,12份(11.01%)用两种方法计数引出的误差相同。新方法计数误差的平均值为(0.071±0.005)×1012/L,明显低于传统方法计数误差的平均值(0.079±0.006)×1012/L,差异有统计学意义(P<0.01)。结论采用新方法在减小牛鲍氏计数板对细胞计数的误差方面可能有一定的作用。

Objective To compare the influence of the classical counting method and the bran-new counting method in Neubauer counting chamber on cell count results.Methods 118anti-coagulated blood samples were selected.The two methods mentioned above were applied respectively to count the number of red blood cells,and results were compared with the standard references.Results Among 109 effective samples,the results of 57samples（52.29%）had more accuracy through using the new counting method,whereas the results of only 40samples（36.70%）had more accuracy through using the conventional counting method,and the results of 12samples（11.01%）had the same errors through using two methods.The average deviation caused by the new method was（0.071±0.005）×1012/L,which was significantly lower than（0.079±0.006）×1012/L（P〈0.01）by the conventional method,the difference was statistically significant（P〈0.01）.Conclusion Adopting the new counting method could have certain effect in the aspect of lessening the error of cell counts in Neubauer counting chamber.