应用ATP生物荧光法调查医院环境物体表面及医务人员手清洁度

Application of ATP bioluminescence assay in survey of cleanliness of hospital environmental object surfaces and health care workers' hands

目的了解医院环境物体表面及医务人员手清洁度。方法采用ATP生物荧光检测法,于2015年1—7月随机抽取某三甲综合医院环境物体表面及医务人员手进行检测并现场反馈干预。结果医院环境物体表面合格率为58.14%(200/344),呼吸机相关物体表面合格率为69.88%(116/166),其中呼吸机气管插管口合格率较低(29.17%);合格率最低的医院环境物体表面为电话(27.27%)。呼吸机相关物体表面合格率:连续使用≥48 h为56.70%(55/97),连续使用<48 h为88.41%(61/69),两者比较差异有统计学意义(χ2=19.26,P<0.01)。医务人员手合格率干预前为34.18%,RLU为(1 033.46±106.20),干预后为85.58%,RLU为(80.46±10.68),干预前后合格率及RLU比较,差异均有统计学意义(均P<0.01)。结论医院动态环境中物体表面及医务人员手污染严重,应用ATP生物荧光检测法检测并进行现场反馈干预,有助于提高环境物体表面清洁度及手卫生依从性。

Objective To investigate cleanliness of hospital environmental object surfaces and hands of health care workers（HCWs）.Methods The adenosine triphosphate（ATP）bioluminescence assay was used to detect object surfaces and hands of HCWs in a hospital,on-the-spot intervention was conducted.Results The qualified rates of hospital environmental object surfaces and ventilator-relevant object surfaces were 58.14%（200/344）and 69.88%（116/166）respectively,the qualified rate of ventilator tracheal intubation site was low（29.17%）;the qualified rate of telephone surfaces was the lowest（27.27%）.The qualified rates of ventilator-relevant object surfaces used continuously for≥48h and〈48 h were 56.70%（55/97）and 88.41%（61/69）respectively,there was significant difference between the two（χ2=19.26,P〈0.01）.The qualified rates of HCWs’ hands before and after intervention were34.18% and 85.58% respectively,relative light unit（RLU）values were（1 033.46±106.20）and（80.46±10.68）respectively,the qualified rates and RLU before and after intervention were both significantly different（both P〈0.01）.Conclusion Contamination of object surfaces and hands’ of HCWs in hospital dynamic environment is serious,ATP bioluminescence detection and on-the-spot intervention is helpful for improving cleanliness of hospital environment object surfaces and HCWs’ compliance to hand hygiene.