电化学传感器测定猪肉中的红霉素残留含量

A novel immunosensor based on chitosan-Prussian blue-graphene nanocomposite and Au nanoparticle for rapid detection of erythromycin

研究了电化学传感器对动物源性食品中红霉素残留的测定。采用电沉积法和共价吸附法将纳米金和壳聚糖/普鲁士蓝/石墨烯复合物修饰到电极表面,构建一种灵敏的电化学免疫传感器。对修饰的电化学传感器进行优化,选取100 ng/m L作为固定抗原的浓度,40 min作为培养时间。对不同浓度红霉素溶液进行检测,得到的线性范围为0.3-1000 mg/m L,检测限为0.15 ng/m L。采用罗红霉素作为干扰物检测电化学传感器的选择性;利用5根电极对同一浓度的红霉素溶液进行测定,其对标准偏差范围为3.8%-5.1%;单支电极连续5次在100 ng/m L的红霉素溶液中测定的标准偏差为4.6%;储存一周后的电化学传感器测定红霉素浓度的标准偏差为4.5%。通过对实际样品（猪肉）的加标测定,回收率范围为86.0%-103.1%。综上所述,该电化学传感器具有很好的选择性、重现性、稳定性和准确性,可以用于猪肉中红霉素残留的检测。

To determine a rapid detection of erythromycin in pork, an immunosensor based on the chitosanPrussian blue-graphene nanocomposite and Au nanoparticle was synthesized in this study. The biosensor exhibits good electrocatalytic behavior to detection of erythromycin and the linear response range from 0.3 ng/m L to 1000 ng/m L with a correlation coefficient of 0.991 and the detection limit of 0.15 ng/m L at the signal to-noise ratio of 3. Some important parameters of the sensor were evaluated and discussed in detail. The optimized concentration of the immobilized antigen and incubation time were 100 ng/m L and 40 min, respectively. The immunosensor also has good selectivity, stability and reproducibility. The recoveries of erythromycin from pork samples ranged from 86.0%-103.1%, which was suitable to test the erythromycin in the pork samples.