DCM猝死与晚期DCM心肌Cx43表达的比较

Expression of myocardial Cx43 in cases of sudden unexpected death with DCM and in patients with serious DCM

目的观察和探讨扩张型心肌病(DCM)猝死与晚期DCM心肌中心肌连接蛋白(connexin,Cx)43表达差异及其意义。方法收集13例DCM猝死者心脏(A组)和5例晚期DCM患者行心脏移植手术切除的心脏(B组),运用免疫组化染色和图像分析技术,检测心肌Cx43阳性表达产物的平均光密度(OD)值和面积(S)值,比较两组间和左右心室间的差异。结果 Cx43阳性表达在A组明显减少,分布不均;在B组表达清晰,主要位于闰盘处。A组与B组心肌Cx43的S值之间的差异有统计学意义(P<0.01),而各组左、右心室肌间相比,差异无统计学意义(P>0.05);心肌Cx43的OD值,在A组与B组之间,以及各组左、右心室肌间差异均无统计学意义(P>0.05)。结论 DCM猝死心肌Cx43表达较晚期DCM心肌明显减少,这种变化可能与DCM患者因心律失常发生猝死有关。

Objective This research was aimed to observe and probe into the expressions and differences of myocardial connexin （Cx）43 between the cases of sudden unexpected death with DCM and patients with a clinical diagnosis of serious DCM. Methods Collect 13 cases of sudden unexpected death with DCM as group A, and 5 patients with serious DCM as group B, then Myocardial Cx43 expression was detected and analyzed by immunohistochemical staining and computerized image analysis. Results Myocardial Cx43 expression was obviously decreased in group A compared to group B. The quantitative data showed that there was significant difference between two groups about Cx43 expressive area （ P 〈 0. 01 ） , but no difference between left and right ventricles in each group itself （P 〉 0. 05 ） and no difference between two groups about average optical density of expression （ P 〉 0. 05 ）. Conclusion Myocardial Cx43 expression was obviously reduced in group A. The alterations of quantity and distribution were probably related to sudden death of the patients with DCM.