隐球菌性脑膜炎患者颗粒溶素的表达及临床意义

Expression and clinical significance of granulysin in cryptococcal meningitis patients

目的探讨隐球菌性脑膜炎患者颗粒溶素(GNLY)的表达和临床意义。方法选择28例隐球菌性脑膜炎患者作为脑膜炎组,另选同期进行健康检查的28例健康人作为健康人组,分离其外周血单个核细胞,并采用免疫印迹法检测其蛋白含量,再使用荧光定量法检测mRNA的含量,分析隐球菌性脑膜炎患者蛋白质含量与其他免疫指标的关系。结果和健康人组相比脑膜炎组GNLY蛋白、mRNA含量明显降低,差异具有统计学意义(P<0.05);脑膜炎组患者的GNLY蛋白质的表达情况和血清中的干扰素(IFN)-γ、免疫球蛋白(Ig)G水平呈正相关,与白细胞介素(IL)-10水平呈负相关。结论颗粒溶素直接影响隐球菌性脑膜炎患者的病情发展,通过测定其水平可为患者的治疗提供理论依据。

Objective To investigate expression and clinical significance of granulysin （GNLY） in cryptoeoccal meningitis patients. Methods There were 28 patients with cryptococcal meningitis as meningitis group, and another 28 healthy people as healthy people group. Mononuclear cell was separated from peripheral blood for detection of protein level by immunoblotting and mRNA content by fluorescence quantification. Relationship between protein level and other immunity indexes was analyzed in cryptococcal meningitis patients. Results Comparing with the healthy people group, the meningitis group had much lower GNLY protein and mRNA levels. The difference had statistical significance （P〈0.05）. Expression of GNLY protein in the meningitis group was positively correlated with serum interferon （IFN）- γ and immunoglobulin （Ig） G levles, and it was negatively correlated with interleukin （IL）-10 level. Conclusion Granulysin show direct influence on disease progression of cryptococcal meningitis, and detection of their contents provides theoretical basis for treatment.